EPA Method 539: Hormones in Drinking Water Using the QTRAP® 6500 LC-MS/MS System

Aug 17, 2016 | Blogs, Environmental / Industrial | 0 comments

Despite government oversight, clean water has become a luxury not afforded some populations. Be it due to physical or economic limits, the World Health Organization (WHO) reports 1.6 million people die each year from diseases attributed to unsafe drinking water, 90 percent of which are under age five.

In other areas such as the United States resources are more plentiful, yet traces of hormones seemingly make their way into the supply via industrial pollution, runoff, agriculture, and landfills. Too much exposure, however, can put consumers at risk of cancer and infertility.

For this reason, the Environmental Protection Agency (EPA), a government agency which protects human health and the environment based on laws passed by Congress, signed the third unregulated monitoring rule (UCMR3) on April 16, 2012. This rule requires monitoring of 30 drinking water contaminants using EPA analytical methods, including EPA Method 539. Within the scope of this regulation, seven hormones are monitored in finished drinking water.

According to the EPA, laboratories using this method have some leniency when it comes to testing but still, they are required to meet minimum reporting levels (MRL). For example, allowances made include changes to LC column, conditions, and standards. Various companies have devised methods according to these standards. However, this one from SCIEX,  not only meets low Method Detection Limits (MDL), it surpasses the UCMR3 reporting limits.

Work in a lab where you test drinking water? You might be interested finding out which pairs of isotopes were monitored to ensure sufficient chromatographic resolution was maintained as described in this technical note, EPA Method 539: Hormones in Drinking Water Using the QTRAP® 6500 LC/MS/MS System and:

  • Get your share of calculations and equations
  • Formulate quality control samples with ongoing results

Want to know more about water testing and the environment?

Discover high-throughput LNP-mRNA integrity profiling

Lipid-based nanoparticles (LNPs) are effective non-viral vectors for delivering messenger RNA (mRNA) products, most notably used for production of vaccines against the recent SARS-CoV-2 pandemic.

Eliminate chick culling with innovative technology

While it sometimes seems questionable whether humanity and modern technology can coexist, technological advances in science can help pave the way to more compassionate business practices.

Using wastewater monitoring to assess exposure to PFAS

Per-and polyfluorinated alkyl substances (PFAS) are known for their water- and grease-resistant properties, which make them useful in many everyday items. In fact, a study from 2020 estimated over 200 “use categories” covering more than 1,400 individual PFAS compounds in commercial products—they are truly all around us. Due to their extensive presence and potentially harmful effects (these effects are still mostly uncertain), exposure to PFAS is a growing concern. Humans and wildlife have been exposed to these chemicals through a variety of routes, including food packaging, drinking water and cleaning products.

Posted by

Craig has worked in the mass spectrometry industry for over 20 years and has been with SCIEX since 2016. As a senior product application specialist, he works with customers to understand their targeted screening workflows and provide solutions using high-resolution accurate mass spectrometry technologies.


Submit a Comment