how integrate separated peaks in multiquant software
I need integrated separated peaks in samples. when I do my calibrate i can integrate two separated peaks only with manual integration, but in this form i can´t apply this integration in all my samples because i need integrate one to one. Could you help me please?
Hi Beatriz, I've messaged the team to help you with this, stay tuned.
From the Support Team:
You can use the parameter "Peak Splitting". You will find this in the integration parameters of the algorithm MQ4. This parameter controls whether a potentially noisy peak is found as one single peak or as two (or more) separate peaks. If the dip between two potential peaks is less than the specified value, then a single peak is found. Otherwise, two peaks are found.
You might try to set a very high value to force the integration of both signals. You have to make sure that the expected RT and the RT half window covers both signals.
Additionally, you have to test different values for noise percentage and baseline subtraction window to achieve desired integration.
Let us know if this helps.
I USED GAUSSIAN SMOOTH WIDTH, EXPECTED RT, RT HALF WINDOWS, PEAK WIDTH, NOISE...ETC BUT I CAN NOT INTEGRATE TWO PEAKS TOO MUCH SEPARATED (FOR EXAMPLE 6,6 MIN ONE OF THEM AT 6,6, MINUTES AND THE OTHER AT 8,4 MINUTES). DO YOU KNOW ANOTHER WAY?
Hi Beatriz, our support team came back and mentioned that this isn't possible. You can not integrate 2 separated signals which are more than 2 minutes away from each other. You can only integrate both peaks separate and add the results together later in the Result Table with the help of a Formula Column and Custom column. Or if you use MultiQuant, a Query and a Custom column.
Another option is to modify the Chromatography, to bring both peaks next to each other, then you can integrate them together.