GEN-MKT-18-7897-A
Member
Once a method has been developed, and good, consistent peak shapes have been obtained for an assay, the Echo® MS system is generally quite consistent. If peak quality deteriorates, consider the following points:
If you see a previously smooth peak become jagged, look at the spray pattern using a small flashlight. If it is flickering, it could indicate plugging of the electrode. Remove the electrode assembly and view it under a magnifying glass. If precipitate is observed on the MS end of the electrode, it can affect the aspiration of the carrier solvent, which can in turn affect the carrier flow and vortex. Remove and clean the electrode in water (most likely to dissolve an organic insoluble precipitate). To clean the electrode, connect it to a high-flow LC pump, if available, for 10–15 minutes; otherwise, soak it in water for 30 minutes. This is more often needed when running high salt or plasma samples. For more information on maximizing the lifetime of the source electrode, please see this post.
RUO-MKT-18-13648-A
Comment below on this article and our team will answer your questions.
You must be logged in to post a comment.
Share this post with your network