GEN-MKT-18-7897-A
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In data-independent acquisition strategies like SWATH acquisition, an expanded mass isolation window is stepped across a mass range covering the mass-to-charge (m/z) distribution of peptides and a full scan MS/MS spectrum is collected at each step. Post-acquisition, high resolution ion chromatograms are extracted for specific fragment ions of the peptides of interest, and these are integrated for quantitation. The information for extraction is stored in a spectral ion library and this contains all the proteins and peptides that you are interested in for the specific proteome you are studying.
There is a combination of acquisition attributes that need to come together to provide highest quality quantitative SWATH acquisition data.
This is why the SCIEX QTOF, TripleTOF and ZenoTOF systems are so powerful for this acquisition technique; because high resolution MS/MS can be acquired at very high speeds, enabling much smaller isolation windows to be used.
RUO-MKT-18-13709-A
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