Hi,
I have an analyte which is resolved into two baseline-separated diastereomer peaks. I need to build a calibration graph using the sum of the peak areas vs concentration. I don’t seem to be able to do this without involving custom calculations – although other manufacturers’ software has functions built in for this situation. Manual integration of both peaks as one peak would be a last resort due to GLP issues.
Is custom calculation the only way forward?
Regards, Andrew
Hi Andrew,
You are right, the best way to do this computation is to use custom columns. We do have a post on this, hopefully this is helpful for you to set up the equations!
https://sciex.com/support/knowledge-base-articles/sciex-os-how-to-sum-concentration-for-isomers_en_us