Author

Andrew Charlton

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September 12, 2023
calibration using sum of peak areas vs concentration

Hi,

I have an analyte which is resolved into two baseline-separated diastereomer peaks. I need to build a calibration graph using the sum of the peak areas vs concentration. I don’t seem to be able to do this without involving custom calculations – although other manufacturers’ software has functions built in for this situation. Manual integration of both peaks as one peak would be a last resort due to GLP issues.

Is custom calculation the only way forward?

Regards, Andrew

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