GEN-MKT-18-7897-A
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When processing SWATH acquisition data, you want to use a reasonably narrow time window to reduce the chance of incorrect peak integrations and to reduce data processing time. There are currently two strategies being employed today to adjust for differences between the retention times in the spectral ion libraries and the retention times observed in the current sample analysis.
Adding RT Calibration protein using SWATH 2.0 in PeakView software
Adding RT Calibration protein in CloudConnect for upload to OneOmics suite
The Retention Time Calibration Protein needs to be created using the CloudConnect software and then new ion library must be loaded into SCIEX Cloud.
Start a CloudConnect session by first logging into your SCIEX Cloud account and then loading both the ion library and a representative data file into the software. The next steps are the same as above.
Once you are satisfied with your RT fit, click Apply. This new ion library that now contains a Retention Time Calibration Protein can now be uploaded into your project folder in SCIEX Cloud for use in processing the data files that match these experimental conditions. Click the Save button, then provide a name for your new library. Select the folder or project that you wish to upload to then click OK to begin the upload.
RUO-MKT-18-6401-A
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