Christie Hunter


General source/gas settings to use when starting method development

Here, we provide a quick reference for the source/gas settings to use at various flow rates with Turbo V, IonDrive Turbo V and OptiFlow Pro ion sources that come with the various SCIEX mass spectrometers. While performing a full source/gas optimization can provide the best sensitivity, the parameters presented here will generally suffice for routine assays. Having proper gas flows and source parameter settings leads to several benefits, including an increase in signal intensity and stability, excellent %CV for analytes and internal standards, lower background, fewer incidences of front-end contamination and reduced instrument downtime due to front-end cleaning and/or repairs.


  1. The new OptiFlow Pro ion source works efficiently at lower ionization voltages (ISVs), typically in the range of 1500v compared to the Turbo V and IonDrive Turbo V ion sources which are typically more efficient at 5000v.
  2. There are no physical adjustments with the OptiFlow Pro ion source.
  3. In atmospheric pressure chemical ionization (APCI) mode, the ISV parameter is replaced with the nebulizing current (NC), which is compound-dependent.
  4. Heater gas (GS2) is not used in APCI mode and is therefore unavailable.
  5. The source temperature (TEM) will need to be higher with a higher flow rate and/or higher aqueous composition in the mobile phase.
  6. Curtain gas (CUR) should be set as high as possible without incurring significant signal-to-noise loss.



  1. For more information on the OptiFlow Pro ion source, refer to Source tuning on the OptiFlow pro ion source.
  2. For method transfer ideas, refer to MRM method transfer from a SCIEX Triple Quad or QTRAP 6500+ system to the SCIEX 7500 system.
  3. Additional information is available in the system user guide for the relevant ion source and at the SCIEX Now Learning Hub at



Join the discussion

Comment below on this article and our team will answer your questions.


Submit a Comment