The ZenoTOF 7600 system is a new QTOF system that launched at ASMS 2021. The key technological advancements in the ZenoTOF 7600 system provide both significantly enhanced MS/MS sensitivity and a new orthogonal fragmentation mode – electron activated dissociation (EAD).  This hybrid collision cell is at the heart of the technological advancements in the ZenoTOF 7600 system.

Learn more about the technology and the applications enabled from these select journal publications.

Zeno trap technology

• Loboda AV, Chernushevich, IV. (2009) A novel ion trap that enables high duty cycle and wide m/z range on an orthogonal injection TOF mass spectrometer.  Journal of the American Society for Mass Spectrometry, 20(7), 1342–1348.
• Chernushevich IV, Merenbloom SI, Liu S, and Nic Bloomfield N. (2017) A W-geometry ortho-TOF MS with high resolution and up to 100% duty cycle for MS/MS. Journal of the American Society for Mass Spectrometry, 28(10), 2143–2150.

Electron activated dissociation (EAD) technology

• Baba T, Ryumin P, Duchoslav E, Chen K, Chelur A, Loyd B, Chernushevich I. (2021) Dissociation of biomolecules by an intense low-energy electron beam in a high sensitivity Time-of-Flight mass Journal of the American Society for Mass Spectrometry, 32(8), 1964-1975. 
• Baba T, Campbell JL, Le Blanc J, Baker P, Hager JW, Thomson BA. (2017) Development of a Branched Radio-Frequency Ion Trap for Electron Based Dissociation and Related Applications. Mass spectrometry (Tokyo, Japan),6(1), A0058.
• Baba, T., Campbell, J. L., Le Blanc, J. C., Hager, J. W., & Thomson, B. A. (2015) Electron capture dissociation in a branched radio-frequency ion trap. Analytical chemistry, 87(1), 785–792. 

Proteome analysis

• Wang Z, Mülleder M, Batruch I, Chelur A, Textoris-Taube K, Schwecke T, Hartl J, Causon J, Castro-Perez J, Demichev V, Tate S, Ralser M. (2022) High-throughput proteomics of nanogram-scale samples with Zeno SWATH DIA.  eLife 11:e83947.
• Wang Z, Tober-Lau P, Farztdinov V, Lemke O, Schwecke T, Steinbrecher S, Muenzner J, Kriedemann H, Sander LE, Hartl J, Mulleder M, Ralser M, Kurth F. (2022)  The human host response to monkeypox infection: a proteomic case series study. EMBO Mol Med 14:e16643.
• Malik AU, Karapetsas A, Nirujogi RS, Chatterjee D, Phung TK Wightman M, Gourlay R, Morrice N, Mathea S, Stefan Knapp S, Alessi DR. (2022) PKC isoforms activate LRRK1 kinase by phosphorylating conserved residues (Ser1064, Ser1074 and Thr1075) within the CORB GTPase domain. Biochem J. 479 (18): 1941–1965.
• Burton JB,  Silva-Barbosa A, Bons, Rose JJ, Pfister K, Simona F, Gandhi T, Reiter K,  Bernhardt O, Hunter CL, Goetzman ES, Sims-Lucas S, Schilling B. (2023) Substantial Downregulation of Mitochondrial and Peroxisomal Proteins during Acute Kidney Injury revealed by Data-Independent Acquisition Proteomics. Preprint BioRxiv.
• Wheeler AM, Orsburn BC, Bumpus NN (2023) Biotransformation of efavirenz and proteomic analysis of P450s and UGTs in mouse, macaque, and human brain-derived in vitro systems. Drug Metab Dispos. DMD-AR-2022-001195. 
• Sun W,  Lin Y, Huang Y,  Chan J,  Terrillon S,  Rosenbaum AI, Contrepois K. (2023) Robust and High-Throughput Analytical Flow Proteomics Analysis of Cynomolgus Monkey and Human Matrices with Zeno SWATH Data Independent Acquisition. Preprint BioRxiv.

Protein/Peptide characterization

• Baba T, Campbell JL. (2015) Capturing Polyradical Protein Cations after an Electron Capture Event: Evidence for their Stable Distonic Structures in the Gas Phase. Journal of the American Society for Mass Spectrometry, 26(10), 1695–1701.
• Baba T, Rajabi K, Liu S, Ryumin P, Zhang Z, Pohl K, Causon J, Le Blanc JCY, Kurogochi M. (2022) Electron Impact Excitation of Ions from Organics on Singly Protonated Peptides with and without Post-Translational Modifications. Journal of the American Society for Mass Spectrometry. 33, 9.
• Kellie JF, Schneck NA, Causon JC, Baba T, Mehl JT, Pohl KI. (2023) Top-Down Characterization and Intact Mass Quantitation of a Monoclonal Antibody Drug from Serum by Use of a Quadrupole TOF MS System Equipped with Electron-Activated Dissociation. J. Am. Soc. Mass Spectrom. 34, 1, 17–26.
• Bons J, Hunter CL, Chupalov R, Causon J, Antonoplis A, Rose J, MacLean B, Schilling B (2023)  Localization and Quantification of Post-Translational Modifications of Proteins Using Electron Activated Dissociation Fragmentation on a Fast-Acquisition Time-of-Flight Mass Spectrometer. J. Am. Soc. Mass Spectrom, e-pub.

Glycans and glycopeptide characterization

• Campbell JL, Baba T, Liu C, Lane CS, Le Blanc J, Hager JW. (2017) Analyzing Glycopeptide Isomers by Combining Differential Mobility Spectrometry with Electron- and Collision-Based Tandem Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 28(7), 1374–1381.
• Baba T, Zhang Z, Liu S, Burton L, Ryumin P, Le Blanc JCY. (2022) Localization of Multiple O-Linked Glycans Exhibited in Isomeric Glycopeptides by Hot Electron Capture Dissociation. J. Proteome Res. 21, 10, 2462–2471. 
• Liu S, Ryumin P, Albanese J, Zhang Z, Baba T. (2023) Analysis of Sialic Acid Linkage in N‑Linked Glycopeptides Using Liquid Chromatography−Electron-Activated Dissociation Time-of-Flight Mass Spectrometry. Anal. Chem. 95, 19, 7458–7467.

Metabolites and small molecule analysis

• Ducati AO, Ruskic D, Sosnowski P, Baba T, Bonner R, Hopfgartner, G. (2021) Improved metabolite characterization by liquid chromatography – Tandem mass spectrometry through electron impact type fragments from adduct ions. Analytica chimica acta, 1150, 338207.
• Huang L, Drouin N, Causon J, Wegrzyn A, Castro-Perez J, Fleming R, Harms A, Hankemeier T. (2023) Reconstruction of Glutathione Metabolism in the Neuronal Model of Rotenone-Induced Neurodegeneration Using Mass Isotopologue Analysis with Hydrophilic Interaction Liquid Chromatography-Zeno High-Resolution Multiple Reaction Monitoring. Anal. Chem. 95, 6, 3255–3266.

Lipid analysis

• Baba T, Campbell JL, Le Blanc JCY, Baker PRS. (2018) Quantitative structural multi-class lipidomics using differential mobility- Electron Impact Excitation of Ions from Organics (EIEIO) mass spectrometry. Journal of lipid research, 59(5), 910-919.
• Baba T, Campbell JL, Le Blanc J, Baker P. (2017) Distinguishing Cis and Trans Isomers in Intact Complex Lipids Using Electron Impact Excitation of Ions from Organics Mass Spectrometry. Analytical chemistry, 89(14), 7307–7315.
• Baba T, Campbell JL, Le Blanc JC, Baker PR. (2016) Structural identification of triacylglycerol isomers using electron impact excitation of ions from organics (EIEIO). Journal of lipid research, 57(11), 2015–2027.
• Baba T, Campbell JL, Le Blanc, JC, Baker PR. (2016) In-depth sphingomyelin characterization using electron impact excitation of ions from organics and mass spectrometry. Journal of lipid research, 57(5), 858–867.
• Campbell JL, Baba T. (2015) Near-complete structural characterization of phosphatidylcholines using electron impact excitation of ions from organics. Analytical Chemistry, 2;87(11), 5837-45.

Oligonucleotides

• Anand P, Koleto M, Kandula DR, Xiong L, MacNeill R. (2021) Novel hydrophilic-phase extraction, HILIC and high-resolution MS quantification of an RNA oligonucleotide in plasma. Bioanalysis, 14(1), epub.
• Karasawa K, Duchoslav E, Takashi Baba T. (2022) Fast Electron Detachment Dissociation of Oligonucleotides in Electron-Nitrogen Plasma Stored in Magneto Radio-Frequency Ion Traps. Anal. Chem. 94, 44, 15510–15517.

RUO-MKT-18-14377-A