The Echo^® MS system is a novel platform for Acoustic Ejection Mass Spectrometry (AEMS) and combines the speed of acoustic sampling with the selectivity of mass spectrometry. This platform has been designed for high throughput analysis of small molecules, metabolites, and peptides. The technology combines Acoustic Droplet Ejection (ADE), an Open Port Interface (OPI) and the SCIEX Triple Quad 6500+ mass spectrometer, learn more about the platform here.

Learn more about the technology and the applications enabled from these select journal publications.

Echo^® MS system technology

• Shou WZ. (2022) Acoustic ejection mass spectrometry: Development, applications, and future perspective. Biomed Chromatogr. 36(2):e5278.
• Zhang H, Liu C, Hua W, Ghislain LP, Liu J, Aschenbrenner L, Noell S, Dirico KJ, Lanyon LF, Steppan CM, West M, Arnold DW, Covey TR, Datwani SS, Troutman MD. (2021) Acoustic Ejection Mass Spectrometry for High-Throughput Analysis. Analytical Chemistry. 93(31):10850-10861.
• Liu C, Van Berkel GJ, Cox DM, Covey TR. (2020) Operational Modes and Speed Considerations of an Acoustic Droplet Dispenser for Mass Spectrometry. Analytical Chemistry. 92(24):15818-15826. 
• Häbe TT, Liu C, Covey TR, Simon RP, Reindl W, Büttner FH, Winter M, Bischoff D, Luippold AH, Runge F. (2020) Ultrahigh-Throughput ESI-MS: Sampling Pushed to Six Samples per Second by Acoustic Ejection Mass Spectrometry. Analytical Chemistry. 92(18):12242-12249.
• Liu C, Van Berkel GJ, Kovarik P, Perot JB, Inguva V, Covey TR. (2021) Fluid Dynamics of the Open Port Interface for High-Speed Nanoliter Volume Sampling Mass Spectrometry. Analytical Chemistry. 93(24):8559-8567.
• Liu C.  (2022) Acoustic ejection mass spectrometry: fundamentals and applications in high-throughput drug discovery. Expert Opin. Drug Discov. 17, 775-787.
• Acoustic Sampling for Mass Spectrometry: Fundamentals and Applications in High-Throughput Drug Discovery, book chapter in High-Throughput Mass Spectrometry in Drug Discovery, 1^st 2023, Wiley. 143-181.

High-throughput screening

• DiRico KJ, Hua W, Liu C, Tucker JW, Ratnayake AS, Flanagan ME, Troutman MD, Noe MC, Zhang H. (2020) Ultra-High-Throughput Acoustic Droplet Ejection-Open Port Interface-Mass Spectrometry for Parallel Medicinal Chemistry. ACS Medicinal Chemistry Letters. 11(6):1101-1110.
• Zhang J, Zhang Y, Liu C, Covey T, Nielsen J, Li S, Weller H, Shou W. (2021) Acoustic Ejection/Full-Scan Mass Spectrometry Analysis for High-Throughput Compound Quality Control. SLAS Technology. 26(2):178-188.
• Wen X, Liu C, Ghislain L, Tovar K, Shah V, Stout SJ, Cifelli S, Satapati S, O’Donnell G, Sheth PR, Wildey MJ, Datwani SS, Covey TR, Bateman KP, McLaren DG. (2021) Direct Analysis from Phase-Separated Liquid Samples using ADE-OPI-MS: Applicability to High-Throughput Screening for Inhibitors of Diacylglycerol Acyltransferase 2. Analytical Chemistry. 93(15):6071-6079.
• Hasegawa T, Imamura RM, Suzuki T, Hashiguchi T, Nomura T, Otsuguro S, Maenaka K, Sasaki M, Orba Y, Sawa H, Sato A, Okabe T, Nagano T, Kojima H. (2022) Application of Acoustic Ejection MS System to High-Throughput Screening for SARS-CoV-2 3CL Protease Inhibitors. Chemical and pharmaceutical bulletin (Tokyo) 70, 199-201.
• Simon RP, Häbe TT, Ries R, Winter M, Wang Y, Fernández-Montalván A, Bischoff D, Runge F, Reindl W, Luippold AH, Büttner FH. (2021) Acoustic Ejection Mass Spectrometry: A Fully Automatable Technology for High-Throughput Screening in Drug Discovery. SLAS Discovery. 26(8):961-973.
• Zacharias AO, Liu C, VanAernum ZL, Covey TR, Bateman KP, Wen X, McLaren DG (2023) Ultrahigh-Throughput Intact Protein Analysis with Acoustic Ejection Mass Spectrometry. J Am Soc Mass Spectrom. 34(1) 4-9.
• Speckmeier E, Pommereau A, Grosser K-C, Mors H, Maier TC, Licher T, Barenz F. (2022) A high-throughput screening assay for mutant isocitrate dehydrogenase 1 using acoustic droplet ejection mass spectrometry, SLAS Discov. 27, 298-305.

• Winter M, Simon RP, Häbe TT, Ries R, Wang Y, Kvaskoff D, Fernández-Montalván A, Luippold AH, Büttner FH, Reindl W (2023) Label-free high-throughput screening via acoustic ejection mass spectrometry put into practice. SLAS Discovery, 28(50), 240-246.
• Liu C. (2023) High-throughput MS for intact protein analysis. Bioanalysis, Aug;15(16):1017-1019.
• McLaren DG, Shah V, Wisniewski T, Ghislain L, Liu C, Zhang H, Saldanha SA. (2021) High-Throughput Mass Spectrometry for Hit Identification: Current Landscape and Future Perspectives. SLAS Discovery 2021, Vol. 26(2) 168–191.
• Silhan J, Klima M, Otava T, Skvara P, et al (2023) Discovery and structural characterization of monkeypox virus methyltransferase VP39 inhibitors reveal similarities to SARS-CoV-2 nsp14 methyltransferase. Nature Communications, 14, 2259.
• Stefek M, Chalupska D, Chalupsky K, Zfarbova M, et al. (2023) Rational Design of Highly Potent SARS-CoV-2 nsp14 Methyltransferase Inhibitors. ACS Omega, 8, 30, 27410-27418.

High-throughput ADME

• Hollenbeck T, Thibodeaux S, White P, Westling L, Chong A, Isbell J. (2020) Acoustic Droplet Ejection and Open Port Interface for Rapid Analysis of Metabolic Stability Assays. Journal of pharmaceutical sciences. 109(11):3285-3291.
• Shou WZ. (2020) Current status and future directions of high-throughput ADME screening in drug discovery. Journal of pharmaceutical analysis. 10(3):201-208. 
• Rimmer MA, Twarog N, Li Y, Shelat AA, Rankovic Z, Yang L. (2023) A High-Throughput Quality Control Method for Assessing the Serial Dilution Performance of Dose–Response Plates with Acoustic Ejection Mass Spectrometry, SLAS Technol. 2023, in press.
• Brehova P, Reznickova E, Skach K, Jorda R, Dejmek M, et al (2023) Inhibition of FLT3-ITD Kinase in Acute Myeloid Leukemia by New Imidazo[1,2-b]pyridazine Derivatives Identified by Scaffold Hopping. J. Med. Chem. 66, 16, 11133-11157.

Platform and extraction optimization

• Guo Y, Forbush M, Covey TR, Ghislain L, Liu C. (2021) High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples. Metabolites. 11(11):789.
• Wagner W, Zhang J, Liu C, Covey TR, Olah TV, Weller HN, Shou WZ (2020) Ultrahigh-throughput and chromatography-free bioanalysis of polar analytes with acoustic ejection mass spectrometry. Anal. Chem. 92, 13525-13531.
• Zhang J, Liu C, Veiga C, Covey T, Shou W, Weller H. (2022) A full scan data review tool to match the speed of acoustic ejection mass spectrometry. LCGC North America, 40, 7, 314-320.

Other

• Droulin N, Liu C, Cox DM, Young JB, et al. (2023) High throughput mass spectrometry assay for early chick gender determination: less than 3s total analysis time per sample, ChemRxiv.

RUO-MKT-18-14377-A