Amanda Bergamin on July 8, 2022 at 2:24 am Hi Christie, Would this be compatible with an EchoMS on a smaller lipid class scale separating on m/z values alone? Would the method parameters carry over to an EchoMS, such as mobile phase and sample prep? Thanks a lot, Amanda Log in to Reply
Christie Hunter on July 9, 2022 at 2:51 pm Great question! So we can do lipidomics using flow injection, as highlighted in this recent work we did with Kevin Williams (UCLA). Quantification of lipids by flow injection analysis and differential mobility spectrometry (https://sciex.com/tech-notes/life-science-research/lipidomics/quantification-of-lipids-by-flow-injection-analysis-and-differen.html) Some classes we do need SelexION device to separate (differential mobility separation, DMS) but others we can do without DMS. So those might be interesting to try. The MRM parameters would carry over for sure, but the source conditions would have to be optimized per the Echo MS system conditions and selected carrier solvent. This tech note would provide ideas for carrier solvent and sample preparation that would keep the lipids happy in solution. To date I haven’t heard of anyone trying lipids on Echo MS system yet, but please let us know if you give this a try and how it works!! Christie Log in to Reply
Ali Khan on December 6, 2022 at 7:42 pm Dear Sir/Mam, I could not able to find MRM file linked to tech note ( https://sciex.com/tech-notes/life-science-research/lipidomics/targeted-profiling-of-lipid-mediators) by Paul Norris. The link for MRM file leads to this site and I could able to find MRM file. Log in to Reply
Christie Hunter on December 6, 2022 at 11:17 pm Please contact Maryam Goudarzi (maryam.goudarzi@sciex.com) and include your instrument model and serial number to request the MRM lists for these methods. She will send you the information! Log in to Reply