Christie Hunter

Member

Get started with these easy-to-implement methods for global lipid profiling

Lipidomics

Quantification of lipids in complex biological samples is challenging and often requires a detailed knowledge of lipids and lipid analysis methods. The inherent complexity of the lipidome means that identifying and quantifying different lipid classes and isobaric species in a single analysis method is difficult and often requires significant expertise and method development time. SCIEX, in collaboration with the lipidomics community, has developed several methods that you can use to begin implementing lipidomics in your lab on your SCIEX Triple Quad or QTRAP systems!

These methods use a helpful sMRM Builder template to streamline the determination of retention times and build Scheduled MRM algorithm acquisition methods.

Comprehensive targeted lipid method

This method uses a targeted time-scheduled MRM acquisition method for the quantification of more than 1900 lipid species. Internal standards, such as the Lipidyzer platform standards kit or the SPLASH Lipidomix standards (Avanti Polar Lipids), can be easily included.

Learn more about this method and download its protocol from this technical note.

 Comprehensive targeted lipid method

This high-throughput, targeted quantitative LC-MS/MS assay was developed to monitor 132 lipid mediators using a single 20-minute MRM assay.

This method has been implemented using either the QTRAP 6500+ system or the SCIEX 7500 system.

 

Metabolomics

Targeted methods for the quantification of plasma metabolites have great utility for many applications, including disease research, food-omics and pharmaceutical development. However, metabolites have diverse structures and therefore their chromatographic and ionization properties vary widely. This variability poses an immense challenge for developing high-throughput, large-scale targeted LC-MS methods for semi-quantitative purposes. SCIEX has developed a targeted MRM assay to address some of these challenges and help you begin to implement targeted metabolomics in your lab.

This method uses a helpful sMRM Builder template to streamline the determination of retention times and build Scheduled MRM algorithm acquisition methods.

 Large metabolomics MRM assay for plasma

A targeted LC-MS/MS assay was developed to profile some of the core metabolites in plasma using MRM transitions for more than 450 metabolites. The method has been tested on both the QTRAP 6500+ system and the SCIEX 7500 system. This method provides the chromatography and MRM transitions to get you begin performing large metabolomics experiments in your lab.

Learn about the power of this method here.

Please contact Maryam Goudarzi (maryam.goudarzi@sciex.com) and include your instrument model and serial number to request the MRM lists for these methods.

 

Join the discussion

Comment below on this article and our team will answer your questions.

2 Comments

  1. Amanda Bergamin

    Hi Christie,
    Would this be compatible with an EchoMS on a smaller lipid class scale separating on m/z values alone?
    Would the method parameters carry over to an EchoMS, such as mobile phase and sample prep?
    Thanks a lot,
    Amanda

    • Christie Hunter

      Great question!
      So we can do lipidomics using flow injection, as highlighted in this recent work we did with Kevin Williams (UCLA).
      Quantification of lipids by flow injection analysis and differential mobility spectrometry (https://sciex.com/tech-notes/life-science-research/lipidomics/quantification-of-lipids-by-flow-injection-analysis-and-differen.html)

      Some classes we do need SelexION device to separate (differential mobility separation, DMS) but others we can do without DMS. So those might be interesting to try.
      The MRM parameters would carry over for sure, but the source conditions would have to be optimized per the Echo MS system conditions and selected carrier solvent. This tech note would provide ideas for carrier solvent and sample preparation that would keep the lipids happy in solution.

      To date I haven’t heard of anyone trying lipids on Echo MS system yet, but please let us know if you give this a try and how it works!!
      Christie

Submit a Comment