We've noticed that with some gel buffer lots, we see a dip in the baseline at 280 nm when analyzing proteins per the SDS-MW protocol. Has anyone experienced this before? We don't always see it and are wondering what causes the dip.
We've noticed that with some gel buffer lots, we see a dip in the baseline at 280 nm when analyzing proteins per the SDS-MW protocol. Has anyone experienced this before? We don't always see it and are wondering what causes the dip.