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Can Resolution value be a zero?
Questions
Questions from customers
ProteinPilot phosphopeptide library and DIA-NN
I have prepared a spectral library for a phosphopeptide enriched sample and I am generating my SWATH samples from similarly enriched samples. The problem is that when I use DIA-NN for the retention time alignment and quant, it doesn’t recognise the terminology of the spectral library annotated by ProteinPilot. DIA-NN recognises Unimod:21 for phosphorylation, but PP uses phospho(Tyr) etc. Other than changing the data dictionary to get around the mismatch, anyone have any suggestions for how I might resolve this? Thank you, Roz
Sequential processing of multiple data-files in ProteinPilot
I would like to use ProteinPilot 5.0.2 to process data-sets containing 16 wiff files acquired from fractionated peptides on a 6600 TripleTOF. A Precision T7910 workstation struggles to process four files in parallel and I would like to be able to queue sequential processing of individual files overnight. I currently use the ‘LC ‘ tab to load and process individual data-files but this leads to parallel processing. Is it possible to generate 16 .group files sequentially?
I want to use the Discovery Quant function at EchoMS
I think EchoMS is powerful tool for high-throughput MS and it would be even better if the Discovery Quant function could be used in SCIEX-OS. I think it will be possible by using the infusion mode.
Please increase the number of files saved as the dispensing log
We found the dispensing log in the folder at C:\ProgramData\SCIEX\EchoMS\Data.
SCIEX Software support for beyond Win10
SCIEX does not support their instruments on windows 10 version 20H2 or above (including Windows 11).
Standard mixture for bare fused silica capillary.
Hi, is there a recommended standard mixture to evaluate the performance of CZE runs in a bare fused capillary with UV/Vis detector? Thanks.
EchoMS OPI Leakage Issue
What is the best way to determine if a recurrent OPI leak during standard operation is due to a faulty/ fouled OPI probe head or a fouled/damaged electrode or poor flow rate optimization?
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