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QTRAP / Triple Quad

QTRAP / Triple Quad systems

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Overcoming N-nitrosamine analysis challenges with mass spectrometry and chromatography solutions

Overcoming N-nitrosamine analysis challenges with mass spectrometry and chromatography solutions

N-nitrosamine analysis has raised significant concerns in the pharmaceutical industry since 2018, when these potential carcinogens were found in several angiotensin II receptor blockers (sartans). Subsequent discoveries in ranitidine and some slow-release metformin medications prompted widespread product recalls. The industry has implemented stricter manufacturing requirements and intensified efforts to evaluate and control N-nitrosamine contamination. To meet stringent regulatory requirements, it is essential to develop effective chromatography and mass spectrometry (MS) methods for N-nitrosamine analysis. This blog addresses the challenges associated with detecting N-nitrosodimethylamine (NDMA) in metformin drug products.

calibration using sum of peak areas vs concentration

Standard peaks are noisy

I am Using Sciex Qtrap 4500 for quantification of nitrosamines (NDMA&NDBA). I am facing issue with higher baseline intensity. When developed the method in APCI the basline was at about 5e4. but now even after cleaning the source (i.e. Changed capillary to new, Curtain plate & Corona needle cleaning, given blank injection overnight ), my base line decreased from 1e6 to 4 e5, but settled at 4 e5 from past 24 hours. can you suggest me how decrease my baseline intensity further to below 1e5. My standard peaks are like noise now.

Using Scheduled Ionization to reduce system ion load for proteomics data acquisition

Using Scheduled Ionization to reduce system ion load for proteomics data acquisition

When analyzing highly complex samples from biological matrices, there can be significant amounts of material that elute in the wash cycle of the LC run, depending on the up-front sample preparation used.  The Scheduled Ionization mode, available in both SCIEX OS...