Biopharma

Biologics characterization pose many challenges due to the diversity of the biomolecules. Learn about the many powerful tools that allow researchers to fully characterize and quantify their biologic drugs, using mass spectrometry and capillary electrophoresis instrumentation.

  • Sorting

  • Filters

7600 EAD and Zeno trap a powerful combination

7600 EAD and Zeno trap a powerful combination

A powerful combination: The Zeno trap and electron activated dissociation (EAD) fragmentation

Sensitivity is a fundamental performance characteristic of a mass spectrometer. Increasingly higher sensitivities are in constant demand in order to characterize and quantify analytes that are at an ever decreasing abundance.

High level method optimization considerations for Echo MS system

High level method optimization considerations for Echo MS system

While an in-depth discussion of method development and optimization for the Echo® MS system is beyond the scope of a community post, here are some points to consider as part of the process: The maximum recommended ion spray voltage for prolonged electrode life is 5000...

High level method optimization considerations for Echo MS system

What to do if you see peak shape deterioration or jagged peaks in your Echo MS system data?

Once a method has been developed, and good, consistent peak shapes have been obtained for an assay, the Echo® MS system is generally quite consistent. If peak quality deteriorates, consider the following points: The viscosity of the sample can affect the peak shape...

High level method optimization considerations for Echo MS system

Tips to maximize electrode lifetime for Echo MS system

While it’s easy to think of the Echo® MS system as an ultrafast LC system in front of the SCIEX Triple Quad 6500+ mass spectrometer, the system operates on fundamentally different principles. For this reason, it requires different routine maintenance to keep it...

Using Bio Tool Kit for interpreting peptide MS/MS data obtained using electron activated dissociation (EAD)

Using Bio Tool Kit for interpreting peptide MS/MS data obtained using electron activated dissociation (EAD)

Peptide MS/MS spectra obtained using either collision induced dissociation (CID) or electron activated dissociation (EAD) can be analyzed using the Peptide Fragments pane in Bio Tool Kit in SCIEX OS Software. First, open the EAD MS/MS spectrum that you want to...

Automatic calibration in minutes for microflow LC with the OptiFlow source on ZenoTOF 7600 system

Automatic calibration in minutes for microflow LC with the OptiFlow source on ZenoTOF 7600 system

The OptiFlow ion source for the SCIEX ZenoTOF 7600 system is equipped with an ESI calibration probe for automatic calibration during microflow experiments (diagram below). The ESI probe is installed orthogonal to the microflow probe in the OptiFlow source and...