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General recommendations when beginning method development Objective: The purpose of this document is to provide a quick reference for transferring MRM-based quantification methods from a SCIEX Triple Quad or QTRAP 6500+ system to a SCIEX 7500 system. While the best...
In data-independent acquisition strategies like SWATH acquisition, an expanded mass isolation window is stepped across a mass range covering the mass-to-charge (m/z) distribution of peptides and a full scan MS/MS spectrum is collected at each step. Post-acquisition,...
To set up a SCIEX high flow source for microflow LC (Turbo V ion source, DuoSpray source or IonDrive Turbo V ion source), first you must replace the wider bore electrodes with more narrow bore hybrid electrodes. Note with the OptiFlow Turbo V ion sources, there are...
While an in-depth discussion of method development and optimization for the Echo® MS system is beyond the scope of a community post, here are some points to consider as part of the process: The maximum recommended ion spray voltage for prolonged electrode life is 5000...
Once a method has been developed, and good, consistent peak shapes have been obtained for an assay, the Echo® MS system is generally quite consistent. If peak quality deteriorates, consider the following points: The viscosity of the sample can affect the peak shape...
While it’s easy to think of the Echo® MS system as an ultrafast LC system in front of the SCIEX Triple Quad 6500+ mass spectrometer, the system operates on fundamentally different principles. For this reason, it requires different routine maintenance to keep it...
The Echo® MS system is a robust platform, and like any other platform, requires some routine maintenance on a regular basis. Here are 3 maintenance steps that can help you ensure consistent, reliable performance: Immediately before use or after switching carrier...
The OptiFlow ion source for the SCIEX ZenoTOF 7600 system is equipped with an ESI calibration probe for automatic calibration during microflow experiments (diagram below). The ESI probe is installed orthogonal to the microflow probe in the OptiFlow source and...
The method of standard addition is a quantitative analysis approach used in situations where matrix effects from complex samples contributes to the analytical signal. This makes it impossible to compare the analytical signal between sample and standard using a...
Contact closure can be used to control external devices that are not directly controlled by SCIEX OS software. A sample batch is first created in the SCIEX OS software for MS acquisition, and then a similar batch is created on an external LC device with the required...
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