Life Science Research

Life science research is the study of living organisms, including human beings, animals and plants, covering subjects in biology, biotechnology, bioinformatics, target discovery and the Omics. Chat with your peers about how to get the most out of your proteomics, metabolomics and lipidomics studies.

  • Sorting

  • Filters

ProteinPilot phosphopeptide library and DIA-NN

ProteinPilot phosphopeptide library and DIA-NN

I have prepared a spectral library for a phosphopeptide enriched sample and I am generating my SWATH samples from similarly enriched samples. The problem is that when I use DIA-NN for the retention time alignment and quant, it doesn’t recognise the terminology of the spectral library annotated by ProteinPilot. DIA-NN recognises Unimod:21 for phosphorylation, but PP uses phospho(Tyr) etc. Other than changing the data dictionary to get around the mismatch, anyone have any suggestions for how I might resolve this? Thank you, Roz

Setting up custom on-column nanoflow calibration for the ZenoTOF 7600 system using PepCalMix

Setting up custom on-column nanoflow calibration for the ZenoTOF 7600 system using PepCalMix

For nanoflow workflows using the ZenoTOF 7600 system and the standard nanoflow interface, on-column calibration is required and can be incorporated automatically into the SCIEX OS software acquisition batch. Here, step-by-step instructions are provided to set up a...

Using Scheduled Ionization to reduce system ion load for proteomics data acquisition

Using Scheduled Ionization to reduce system ion load for proteomics data acquisition

When analyzing highly complex samples from biological matrices, there can be significant amounts of material that elute in the wash cycle of the LC run, depending on the up-front sample preparation used.  The Scheduled Ionization mode, available in both SCIEX OS...