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Life Science Research

Life science research is the study of living organisms, including human beings, animals and plants, covering subjects in biology, biotechnology, bioinformatics, target discovery and the Omics. Chat with your peers about how to get the most out of your proteomics, metabolomics and lipidomics studies.

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The costly consequences of unplanned downtime

The costly consequences of unplanned downtime

Unplanned downtime is a formidable adversary that businesses across various industries strive to minimize. Defined as the unexpected interruption of regular operations, unplanned downtime can wreak havoc on productivity, profitability and customer satisfaction. In this article, we delve into the causes of unplanned downtime, its far-reaching consequences and strategies to mitigate its impact.

ProteinPilot phosphopeptide library and DIA-NN

ProteinPilot phosphopeptide library and DIA-NN

I have prepared a spectral library for a phosphopeptide enriched sample and I am generating my SWATH samples from similarly enriched samples. The problem is that when I use DIA-NN for the retention time alignment and quant, it doesn’t recognise the terminology of the spectral library annotated by ProteinPilot. DIA-NN recognises Unimod:21 for phosphorylation, but PP uses phospho(Tyr) etc. Other than changing the data dictionary to get around the mismatch, anyone have any suggestions for how I might resolve this? Thank you, Roz

Optimized rolling collision energy curves for IDA and SWATH DIA for peptides

Setting up custom on-column nanoflow calibration for the ZenoTOF 7600 system using PepCalMix

For nanoflow workflows using the ZenoTOF 7600 system and the standard nanoflow interface, on-column calibration is required and can be incorporated automatically into the SCIEX OS software acquisition batch. Here, step-by-step instructions are provided to set up a...

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