Data acquisition

Data acquisition

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Using the Skyline / Panorama AutoQC tools for continuous monitoring your TripleTOF system performance

Using the Skyline / Panorama AutoQC tools for continuous monitoring your TripleTOF system performance

The Skyline team has developed a nice pipeline for evaluation of LC-MS/MS performance over time for MS systems in the research lab environment.  To help you use this solution, we have set up two Skyline Documents that you can use for continuous system monitoring....

What is variable window SWATH acquisition and why does it improve results quality?

What is variable window SWATH acquisition and why does it improve results quality?

To continue to improve the quality of the SWATH acquisition data that one can achieve in complex matrices, we are working on ways that we can decrease the size of the Q1 window, while still maintaining full mass range coverage and optimal cycle times.  One great way...

What is variable window SWATH acquisition and why does it improve results quality?

What is SWATH acquisition and what are the critical acquisition attributes?

In data-independent acquisition strategies like SWATH acquisition, an expanded mass isolation window is stepped across a mass range covering the mass-to-charge (m/z) distribution of peptides and a full scan MS/MS spectrum is collected at each step. Post-acquisition,...

Adapting a SCIEX high flow source for microflow LC

Troubleshooting low flow LC – Loss of early eluting peptides during trap-elute workflow

Symptom: Early eluting peptides are missing (based on monitoring PepCalMix LC-MS test or observing a change in the shape of the proteome TIC). Test: Use PepCalMix LC-MS test to monitor binding of early eluting peptides. There are a number of very hydrophilic peptides...

High level method optimization considerations for Echo MS system

High level method optimization considerations for Echo MS system

While an in-depth discussion of method development and optimization for the Echo® MS system is beyond the scope of a community post, here are some points to consider as part of the process: The maximum recommended ion spray voltage for prolonged electrode life is 5000...