Optimizing Performance

Optimizing Performance

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Using the Skyline / Panorama AutoQC tools for continuous monitoring your TripleTOF system performance

Using the Skyline / Panorama AutoQC tools for continuous monitoring your TripleTOF system performance

The Skyline team has developed a nice pipeline for evaluation of LC-MS/MS performance over time for MS systems in the research lab environment.  To help you use this solution, we have set up two Skyline Documents that you can use for continuous system monitoring....

sMRM Pro Builder template tutorial

sMRM Pro Builder template tutorial

The sMRM Pro Builder template is an Excel-based tool that can help you implement large panels of analytes in your lab. The Excel sheet will take your preliminary experimental results and compute retention times, retention time window widths and dwell time weighting to optimize your targeted assay.

Using the Skyline / Panorama AutoQC tools for continuous monitoring your TripleTOF system performance

Files for running the SWATH acquisition performance kit – ion library, performance trackers

The FASTA file required for use with the SWATH acquisition performance kit installs with ProteinPilot software or is available within OneOmics suite. Download this SWATH acquisition ion library for use with the SWATH acquisition performance kit Human2D_SOP_PepCalMix_...

Troubleshooting low flow LC – LC-MS peak area is lower than expected