Lipidomics

Collaborate with your peers. Pose and answer questions or review responses about the use of mass spectrometry and the analysis of lipids here. Discover how to get started on lipid sample preparation techniques and results.

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What is the Zeno threshold and what does it do?

What is the Zeno threshold and what does it do?

With the introduction of the Zeno trap on the ZenoTOF 7600 system, sensitivity improvements on the order of 4 to 25x can be seen for MS/MS analysis, because of the optimization of duty cycle.1 When concentrations are low and peak intensities are limited, this bump in...

Why Study Lipids?

Why Study Lipids?

I had an opportunity to follow up with Steven M Watkins, Ph.D. to talk about the importance of studying lipids in disease. Steve has been working in the lipids field for over 20 years and is one of the foremost experts in lipid biology. Steve founded Lipomics in 2000, an early metabolomics company focused on quantitative lipidomics and had followed that company through a series of changes that led to its involvement in the clinical diagnostic development and global metabolomics. Steve authored over 70 peer-reviewed publications including several book chapters on lipids and lipid metabolism. His presentations on this topic are fascinating and very informative, so I wanted to capture some of his thinking here!

Simplify and Improve the Analysis of Lipid Molecular Species Using SelexION Technology

Simplify and Improve the Analysis of Lipid Molecular Species Using SelexION Technology

Analyzing lipids can be tricky. Lipids tend to have a wide variety of chemical structures, and this makes it virtually impossible to find one LC method that can resolve all lipid species. Therefore, multiple LC methods are often used. Also, there is widespread isobaric and isomeric overlap between different lipid classes and species, therefore separating lipid compounds, using an orthogonal separation prior to mass analysis is important.