Methods for electrode cleaning for Echo MS systems

Jun 3, 2025 | Blogs, Echo® MS+ system, Pharma | 0 comments

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Depending on the samples that you are running on the system, it is possible for the Echo MS electrode to become dirty or occluded over time. Below are two different cleaning strategies that will be helpful for you to maintain your system and keep your electrodes running well.

Quick cleaning protocol

This protocol is easy to perform between running your sample plates.

From the Maintenance menu of the Echo MS direct control, select OPI Port Access then Lift to provide access to the top of the OPI port.
Disconnect electrode by loosening the finger tight red nut and pulling straight up
Place the OPI port end of the electrode into solvent. With the MS system still active, the draw created from the GS1 flow will draw solvent through the OPI into the MS source, turn Spray voltage to 0.
Wash with Milli-Q water for 1 min
Wash with 20% Acetonitrile with 0.1 M Formic Acid (0.4% FA) for 20 min
- Drawing speed is about 450 µL/min based on the consumption of 20% Acetonitrile with 0.1 M FA
Wash with Milli-Q water for 1 min
Reattach the electrode to the system and tighten the red nut finger tight.
Restart the mobile phase flow and check the Maximum Supported Flow (MSF)

Intensive cleaning protocol

This protocol is an offline protocol that can be used periodically for a more intensive wash of the electrode or to recover electrodes that are suffering from reduced flow rates.

Connect the OPI electrode to a small union (1/16 to 1/32) , IDEX Part # P-881 )

Place the electrode end of the capillary in a waste vessel capable of holding 200 mL or more
Flow at least 200 ml of 50% methanol through the electrode at a flow rate of 5 – 8 mL/min using an HPLC pump
Test on Echo MS system for droplet linearity, reproducibility, and peak shape
If storing the recovered electrode flush with 100% methanol before storing.

Some electrodes may not be able to be recovered but we have had good success with this method.

Journal publications using the Echo® MS (+) system

The Echo® MS+ system is a novel platform for Acoustic Ejection Mass Spectrometry (AEMS) and combines the speed of acoustic sampling with the selectivity of mass spectrometry. This platform has been designed for high throughput analysis of small and large molecules. The technology combines Acoustic Droplet Ejection (ADE), an Open Port Interface (OPI) and could be coupled with the SCIEX Triple Quad 6500+ system or the ZenoTOF 7600 system.

Technical notes using the Echo® MS+ system

The Echo® MS+ system comprises of an open-port interface (OPI) and acoustic droplet ejection (ADE) module which could be coupled with a mass spectrometer. The mass spectrometer could either be a SCIEX Triple Quad 6500+ system or the ZenoTOF 7600 system. This non-liquid chromatography based; high-throughput screening platform enables rapid analysis of compounds at speeds of up to 1 sample/second.

Bulletproof your clinical laboratory with rugged and reliable LC-MS/MS instrumentation (Clinical diagnostic blog)

The ability to consistently achieve reproducible results on many complex samples across multiple days is critical to a routine clinical laboratory. Laboratories relying on analytical instrumentation require stability and robustness to perform a variety of screening and confirmatory assays with confidence. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become the preferred analytical method in the clinical laboratory to reliably perform clinical testing as it provides best-in-class performance and reliability for the most challenging assays. LC-MS/MS offers the required levels of sensitivity and specificity for the detection and quantitation of molecules from complex biological samples, helping laboratories deliver highly accurate data for a variety of clinically relevant analytes across a wide range of assays.

Posted by

John Gibbons

Trained and working in Organic Synthesis I started using mass spectrometry to aid in my synthesis work. As my interest in mass spectrometry grew, I change roles several times within the pharmaceutical industry to be able to focus on its use and applications. I joined SCIEX in 2006, initially as a demo chemist, then with R&D, primarily focused on Analyst, and eventually SCIEX OS software development. More recently I switched to technical product management for Echo MS systems.

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