GEN-MKT-18-7897-A
Apr 28, 2026 | Biopharma, BioPhase 8800 system, Blogs | 0 comments
CE‑SDS remains a cornerstone assay for characterizing fragmentation, aggregation, and product‑related impurities in therapeutic proteins. UV detection has been the long‑standing standard. However, it frequently struggles with baseline noise, limited sensitivity for minor fragments, and subjective integration.
Native fluorescence detection (NFD) strengthens CE‑SDS performance by improving both detection sensitivity and interpretability.
High sensitivity without labeling
NFD leverages intrinsic tryptophan fluorescence to detect proteins label‑free, avoiding the variability and workflow burden of dye‑labeling steps.
Technical note data show:
For scientists managing stability programs or performing forced degradation studies, this sensitivity can reveal early‑stage degradation signatures that UV cannot reliably resolve.
Clearer, more stable baselines for better integration
Typical UV electropherograms show baseline drifting due to buffer absorbance and gel heterogeneity.
The NFD baseline, however, is consistently flat, improving:
These benefits reduce manual reprocessing and strengthen data defensibility across development stages.
Multi-capillary throughput for faster studies
The BioPhase 8800 system processes 8 samples in parallel, expanding CE‑SDS throughput without requiring additional instruments or longer sequences.
High‑throughput CE‑SDS paired with NFD’s sensitivity provides a strong foundation for data‑driven therapeutic development.
A balanced upgrade path from UV and LIF
NFD complements existing detection modes:
This offers scientists flexibility while building long‑term method robustness.
Explore the CE‑SDS NFD data
For deeper review, check out the resources in our NFD solutions hub >
Explore why capillary electrophoresis remains essential for high resolution, confident analytics across modern biopharma modalities, from proteins to mRNA and gene therapies
Capillary electrophoresis (CE) is not confined to a single point in the drug development lifecycle. Its value comes from the ability to deliver high‑resolution, reproducible separations that remain relevant as analytical questions evolve, from early discovery through late‑stage development and lot release.
Finding the right information shouldn’t slow you down. Whether you’re troubleshooting your mass spec, learning something new, or optimizing performance, access to the right resources at the right moment makes all the difference.
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