Kirsten Craven

Metabolite identification and peace of mind

Feb 13, 2024 | Blogs, LC, Pharma, QTRAP / Triple Quad, ZenoTOF 7600 system | 0 comments

Read Time: 3 minutes

Managing metabolite identification (Met ID) studies is challenging, so what is at the top of your priority list as you plan the year ahead? Ensuring you have the data needed to manage product safety, meeting deadlines, staff recruitment and training, maintaining compliance, capital expenses, or something else?

At SCIEX, we aim to design solutions that solve these real-world challenges, to make the process more efficient. Imagine the advantages of unambiguous metabolite identification by liquid chromatography-mass spectrometry (LC-MS), even for challenging analytes such as phase II confirmation of glucuronide metabolites.

How can we help?

Manage product safety

Help ensure product safety with complete structural elucidation—even for challenging analytes such as phase II metabolites—with electron activated dissociation (EAD). The ZenoTOF 7600 system can collect EAD and collision-induced dissociation (CID) MS/MS spectra in a single LC-MS/MS experiment.

Meet deadlines

Robust and reliable instruments allow for predictable scheduling and on-time project completion. When needed, our systems are supported by highly respected services and backed by the SCIEX Now support network to ensure that service calls are quickly reported and tracked.

Efficient staff recruitment and training

Streamline new-hire training with knowledge-base articles available on demand from the SCIEX Now Learning Hub. SCIEX OS software is designed to be easy to learn and use for data collection, processing and reporting within a single platform.

Regulatory requirements

SCIEX OS software is compliant-ready to help ease the burden of regulatory activities, including managing user access levels and to reduce the potential for human error.

Capital expense

Invest only in what you need, so you don’t have to spend money on features and system performance that are unnecessary for your samples.

Which system is right for you?

Routine metabolite identification

Larger batches of samples with analytes that typically fragment well using collision-induced dissociation (CID) enable confident identification of the site of metabolism without additional confirmatory testing. In this situation where priorities are typically ease of use and a robust system that does the job day in and day out, SCIEX recommends using the ExionLC AE system coupled to the X500R QTOF system, SCIEX OS software and maintenance with SCIEX service and support.

The workflow described in the technical note Rapid soft spot analysis using a routine biotransformation solution (sciex.com) demonstrates the performance you can expect from this system.

Complex metabolite identification

When clear identification of the site of metabolism is not possible using CID, a secondary analytical technique, such as nuclear magnetic resonance (NMR), is often required to meet regulatory requirements. Since this approach can be both time-consuming and expensive, an LC-MS system with more analytical power is ideal.

The ZenoTOF 7600 system offers electron activated dissociation (EAD) as a complementary fragmentation option to CID. EAD typically fragments the metabolite in different locations, which can provide the additional information required for clear identification of the site of metabolism from a single analytical technique.

SCIEX recommends using the ExionLC AE system coupled to the ZenoTOF 7600 system, SCIEX OS software and maintenance with SCIEX service and support.

The workflow described in the technical note Enhanced drug metabolite identification using collision induced dissociation (CID) and electron-activated dissociation (EAD) (sciex.com) is a useful example of this solution.

Learn more

For more information on the options SCIEX offers for Met ID, please speak to your account manager or visit our web page on Comprehensive Metabolite Identification | SCIEX

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Posted by

Kirsten Craven

Kirsten Craven is the Senior Global Marketing Manager for Pharma global strategic marketing at SCIEX. In this role, she manages strategic marketing for the pharmaceutical industry. Kirsten spent the first part of her career working in laboratories across multiple industries before moving into product management, and most recently pharma marketing.

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