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Managing metabolite identification (Met ID) studies is challenging, so what is at the top of your priority list as you plan the year ahead? Ensuring you have the data needed to manage product safety, meeting deadlines, staff recruitment and training, maintaining compliance, capital expenses, or something else?
So, you need a new liquid chromatography-mass spectrometry (LC-MS) system for your metabolite identification (metID) studies, and you are not sure which option is right for you. This blog provides an overview of the metID solutions offered by SCIEX, so you can make the best decision for your organization.
Different LC options are supported in SCIEX OS software and Analyst software. Those that communicate through ethernet differ in their default IP addresses. In this community post, we will detail the LAN setup, configuring the IP address and subnet mask for LC...
To set up a SCIEX high flow source for microflow LC (Turbo V ion source, DuoSpray source or IonDrive Turbo V ion source), first you must replace the wider bore electrodes with more narrow bore hybrid electrodes. Note with the OptiFlow Turbo V ion sources, there are...
Symptom: LC-MS peptide peak are is smaller than expected in PepCalMix Test Test: Use PepCalMix Infusion tests to determine if it is a problem with LC or MS Solution: First perform the SCIEX recommended performance tests using infusion on your MS system. If these...
Symptom: The observed retention times of the peptides eluting are compressed, are not as spread out as expected, suggesting the elution gradient is compressed Test: Use PepCalMix LC-MS test to check the peptide elution time and spread Solution: Make sure you are...
Symptom: Early eluting peptides are missing (based on monitoring PepCalMix LC-MS test or observing a change in the shape of the proteome TIC). Test: Use PepCalMix LC-MS test to monitor binding of early eluting peptides. There are a number of very hydrophilic peptides...
Using the dual gradient system, the nanoLC 425 system can be configured such that the trap can be washed to waste rather than through the column and into the mass spectrometer. This workflow can be performed with either nanoflow or microflow chromatography. Figure 1....
Contact closure can be used to control external devices that are not directly controlled by SCIEX OS software. A sample batch is first created in the SCIEX OS software for MS acquisition, and then a similar batch is created on an external LC device with the required...
SCIEX OS software can support external devices that are not under direct control by using contact closure. Contact closure enables synchronization of the SCIEX OS software controlled device (the MS) and the external device (the LC in this case), ensuring that...
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