Troubleshooting low flow LC – LC-MS peak area is lower than expected

Symptom: LC-MS peptide peak are is smaller than expected in PepCalMix Test

Test: Use PepCalMix Infusion tests to determine if it is a problem with LC or MS

Solution:

First perform the SCIEX recommended performance tests using infusion on your MS system.
If these standard infusion tests have all passed, then the problem could be related to LC.
- Check if autosampler picks up the correct volume. One easy way to do this is to fill an autosampler vial with a volume you want to inject plus 2 µ Then after pickup, check to see if there is only 2 µL remaining in your vial.
- Check for flow path leaks, both the gradient and trap flow paths
- Replace trap and/or column
- Prepare fresh solvents, if solvents have been in use for >3months
- Prepare fresh PepCalMix, ensure you are using the recommended dilution solution recommended in the protocol

RUO-MKT-18-13872-A

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