Kirsten Craven

Metabolite identification and peace of mind

Feb 13, 2024 | Blogs, LC, Pharma, QTRAP / Triple Quad, ZenoTOF 7600 system | 0 comments

Read Time: 3 minutes

Managing metabolite identification (Met ID) studies is challenging, so what is at the top of your priority list as you plan the year ahead? Ensuring you have the data needed to manage product safety, meeting deadlines, staff recruitment and training, maintaining compliance, capital expenses, or something else?

At SCIEX, we aim to design solutions that solve these real-world challenges, to make the process more efficient. Imagine the advantages of unambiguous metabolite identification by liquid chromatography-mass spectrometry (LC-MS), even for challenging analytes such as phase II confirmation of glucuronide metabolites.

How can we help?

Manage product safety

Help ensure product safety with complete structural elucidation—even for challenging analytes such as phase II metabolites—with electron activated dissociation (EAD). The ZenoTOF 7600 system can collect EAD and collision-induced dissociation (CID) MS/MS spectra in a single LC-MS/MS experiment.

Meet deadlines

Robust and reliable instruments allow for predictable scheduling and on-time project completion. When needed, our systems are supported by highly respected services and backed by the SCIEX Now support network to ensure that service calls are quickly reported and tracked.

Efficient staff recruitment and training

Streamline new-hire training with knowledge-base articles available on demand from the SCIEX Now Learning Hub. SCIEX OS software is designed to be easy to learn and use for data collection, processing and reporting within a single platform.

Regulatory requirements

SCIEX OS software is compliant-ready to help ease the burden of regulatory activities, including managing user access levels and to reduce the potential for human error.

Capital expense

Invest only in what you need, so you don’t have to spend money on features and system performance that are unnecessary for your samples.

Which system is right for you?

Routine metabolite identification

Larger batches of samples with analytes that typically fragment well using collision-induced dissociation (CID) enable confident identification of the site of metabolism without additional confirmatory testing. In this situation where priorities are typically ease of use and a robust system that does the job day in and day out, SCIEX recommends using the ExionLC AE system coupled to the X500R QTOF system, SCIEX OS software and maintenance with SCIEX service and support.

The workflow described in the technical note Rapid soft spot analysis using a routine biotransformation solution (sciex.com) demonstrates the performance you can expect from this system.

Complex metabolite identification

When clear identification of the site of metabolism is not possible using CID, a secondary analytical technique, such as nuclear magnetic resonance (NMR), is often required to meet regulatory requirements. Since this approach can be both time-consuming and expensive, an LC-MS system with more analytical power is ideal.

The ZenoTOF 7600 system offers electron activated dissociation (EAD) as a complementary fragmentation option to CID. EAD typically fragments the metabolite in different locations, which can provide the additional information required for clear identification of the site of metabolism from a single analytical technique.

SCIEX recommends using the ExionLC AE system coupled to the ZenoTOF 7600 system, SCIEX OS software and maintenance with SCIEX service and support.

The workflow described in the technical note Enhanced drug metabolite identification using collision induced dissociation (CID) and electron-activated dissociation (EAD) (sciex.com) is a useful example of this solution.

Learn more

For more information on the options SCIEX offers for Met ID, please speak to your account manager or visit our web page on Comprehensive Metabolite Identification | SCIEX

3 reasons ZT Scan DIA is changing the game in metabolomics

For years, metabolomics researchers have faced a frustrating reality: modern high-resolution mass spectrometers can detect tens of thousands of molecular features, yet only a fraction can be confidently identified. The challenge is no longer finding molecules. It is generating the high-quality MS/MS data needed to confidently assign structures and extract meaningful biological insights.

ADC analytics FAQs: Answering the most common questions about ADC characterization

Antibody-drug conjugates (ADCs) have emerged as one of the fastest-growing classes of biotherapeutics, combining the targeting specificity of monoclonal antibodies with the potency of cytotoxic payloads. However, this unique architecture also introduces significant analytical complexity. From drug-to-antibody ratio (DAR) distributions and charge variants to conjugation sites and structural modifications, ADCs present multiple, interconnected sources of heterogeneity that must be understood to ensure product quality and performance.

Sterile but toxic: Cereulide detection in food matrices

In a recent webinar, Ming Gao, Ph.D., Senior Research Chemist at Mérieux NutriSciences, explored the growing challenge of cereulide contamination in food products and the analytical strategies needed to detect this heat-stable toxin at trace levels. The session examined how LC-MS/MS can help laboratories overcome the limitations of traditional microbiological screening and meet emerging regulatory requirements.

Posted by

Kirsten Craven

Kirsten Craven is the Senior Global Marketing Manager for Pharma global strategic marketing at SCIEX. In this role, she manages strategic marketing for the pharmaceutical industry. Kirsten spent the first part of her career working in laboratories across multiple industries before moving into product management, and most recently pharma marketing.

0 Comments