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Dec 1, 2015 | Blogs, Life Science Research, Proteomics | 0 comments
SWATH® Acquisition: On the Forefront of HIV-1 Research
World AIDS Day is held on the 1st December each year and is an opportunity for people worldwide to unite in the fight against HIV, show their support for people living with HIV, and to commemorate people who have died. World AIDS Day was the first ever global health day, held for the first time in 1988.
Source: http://www.worldaidsday.org/about
A research team at the University of Nebraska Medical Center (UNMC) is using SWATH Acquisition to advance a host-oriented antiviral strategy that targets the biomolecules required for viral replication.
Using SWATH for quantitative proteomics together with bioinformatic analyses to identify host proteins, the team quantified the expression of 3,608 proteins in uninfected and HIV-1-infected monocyte-derived microphages.
Of these, they found that 420 were significantly altered upon HIV-1 infection, and the findings highlighted a novel set of proteins and processes that are involved in the host response to HIV-1 infection.
Journal of Proteomics Research, 2014, April 4; Drs. P. Ciborowski, N. Haverland, H. Fox, University of Nebraska Medical Center or VIEW the webinar (May 2014) by Drs. Pawel Ciborowski and Nicole Haverland
In this informative presentation, you’ll learn:
In monoclonal antibody (mAb) development, assessment of purity and integrity of the protein in question is critical. CE‑SDS is the gold standard assay and is routinely run from analytical development through QC and lot release. It’s trusted because it consistently delivers quantitative, size‑based insight into purity and fragmentation, and it fits naturally into regulated environments.
In drug discovery and development, Metabolite Identification (Met ID) plays a critical role in understanding biotransformation pathways, ensuring safety, and meeting regulatory requirements. Advanced mass spectrometry techniques have revolutionized this process, particularly through electron-based fragmentation methods such as Electron Activated Dissociation (EAD) and Electron Transfer Dissociation (ETD). While both techniques leverage electron interactions to generate informative fragment ions, they differ significantly in mechanism, performance, and suitability for Met ID workflows.
In analytical laboratories, performance is not optional. Whether supporting regulated pharmaceutical workflows, high-throughput CRO operations, clinical reporting, or food and environmental testing, your mass spectrometry and capillary electrophoresis systems are critical to productivity, compliance, and scientific confidence.
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