GEN-MKT-18-7897-A
Feb 18, 2021 | Blogs, Food / Beverage | 0 comments
Read time: 3 minutes
A recent webinar from SCIEX and New Food, presented by Jianru Stahl-Zeng of SCIEX, outlined some of the capabilities of the new SCIEX Triple Quad™ 7500 LC-MS/MS System – QTRAP® Ready. In particular, it highlighted the ability of the SCIEX 7500 System to accurately quantify 700 pesticide residues across 10 different food matrices at ultra-low trace levels. During the webinar, attendees posed various questions. Here, we share the top 5 of these questions, along with their answers.
1. How did you set up the OptiFlow® Pro Ion Source for the assay? Is it any different from the Turbo V™ Ion Source? The OptiFlow Pro Ion Source typically requires lower carrier gas (Gas 1) pressure and ion spray voltage than the Turbo V Ion Source. This assay uses 1500 V for the ion spray and Gas 1 at 35.
2. What is the dynamic range of the SCIEX 7500 System? The SCIEX 7500 System offers a linear dynamic range spanning up to 6 orders of magnitude.
3. How are data points related to cycle time and dwell time? How does this affect the peak shape? You will get sufficient data points to ensure your quantification quality when your instrument is fast enough and the cycle time is not too long. A shorter cycle time and dwell time equals more data points. Enough data points are essential for reproducible peak shape. With advanced sMRM algorithm, you are able to run so many compounds with shorter cycle times.
4. How long does it take to switch polarity from positive to negative mode? How does this impact my assay? And what is the dwell time for each multiple reaction monitoring (MRM) transition in the positive and negative modes? The SCIEX 7500 System can switch polarity in as little as 5 ms. In our screening assay, where we strive to get a large number of data points, we used 5 ms. In the quantitative assay, where reproducibility and consistent peak integration are important, we used 15 ms. Given the target cycle time of 350 ms, the longer polarity switching time had minimal impact on the number of points across each peak. Dwell time for each transition is calculated by SCIEX OS Software and depends on factors such as your liquid-chromatography (LC) separation, the number of coeluting peaks and the predefined cycle time. This is the same for both positive and negative modes.
5. Along with signal increase, does the larger orifice also affect noise in the system? With a larger orifice, the background noise will increase for some of the analyte signals as you are sampling more ions from the source stream. However, the analyte-specific signal increases will outpace the background noise and deliver meaningful signal-to-noise gains from the SCIEX 7500 System.
For more information on the capabilities of the SCIEX 7500 System, and to access related content, catch the webinar on demand.
RUO-MKT-18-12793-A
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has gained significant attention in the clinical laboratory due to its ability to provide best-in-class sensitivity and specificity for the detection of clinically relevant analytes across a wide range of assays. For clinical laboratories new to LC-MS/MS, integrating this technology into their daily routine operations may seem like a daunting task. Developing a clear outline and defining the requirements needed to implement LC-MS/MS into your daily operations is critical to maximize the productivity and success of your clinical laboratory.
In today’s rapidly evolving food industry, the role of food testing laboratories has never been more critical. Ensuring the safety, quality, and authenticity of food products is paramount, and this responsibility falls heavily on the shoulders of laboratory managers. The economics of food testing—encompassing everything from high-throughput pesticide screening to advanced research on alternative protein sources—plays a pivotal role in shaping the operational efficiency and financial health of these laboratories.
Imagine having a tech expert at your fingertips to solve computer issues or a fitness trainer guiding you through workouts from the comfort of your home. In today’s fast-paced world, the ability to provide and receive service and support remotely is no longer a luxury but a necessity. Whether it’s troubleshooting a software issue, repairing a device, offering customer assistance, or enjoying the convenience of telehealth as a private individual, remote capabilities have revolutionized how businesses operate and how individuals get help
Posted by
You must be logged in to post a comment.
Share this post with your network