Computing protein confidence with improved accuracy by reassessing peptide confidence during protein grouping by Christie Hunter | Aug 5, 2021 | Data processing, Life Science Research, ProteinPilot, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemProteinPilot software 4.0 and higher releases include a new method for calculating protein confidences that improves reliability at the end of protein lists. Figure 1 shows a simulated example that demonstrates how more accurate protein confidence is computed. Figure...
Unused Prot Score in ProteinPilot software by Christie Hunter | Aug 5, 2021 | Data processing, Life Science Research, ProteinPilot, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemThere are 2 key protein scores that are computed in ProteinPilot™ Software: Total ProtScore and Unused ProtScore. Total ProtScore is simply a sum of all the peptide evidence related to a given protein, while Unused ProtScore reflects the amount of total, unique...
How does multi-precursor searching work in ProteinPilot software 5.0? by Christie Hunter | Aug 5, 2021 | Data processing, Life Science Research, ProteinPilot, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemIn highly complex proteomics data, some percentage of MS/MS spectra is chimeric due to the transmission of more than 1 precursor through the MS isolation step, even with a 1 Da window like the one used on TripleTOF systems. The ProteinPilot software 5.0 release...
Isoform-specific quantification in ProteinPilot software by Christie Hunter | Aug 5, 2021 | Data processing, Life Science Research, ProteinPilot, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemDuring a ProteinPilot software search, the Pro Group algorithm assembles all the peptides into their appropriate proteins. Before the protein ratios are computed, the peptides are automatically annotated according to specific properties that make them more or less...
Understanding the Paragon algorithm for database searching in ProteinPilot software by Christie Hunter | Aug 5, 2021 | Data processing, How-to videos, Life Science Research, ProteinPilot, Proteomics, QTOF, ZenoTOF 7600 systemThis short video provides an overview of the Paragon algorithm, a novel search engine that enables a researcher to search and identify a broad range of proteins and post-translational modifications in a single search, without increasing false positives and not...