Christie Hunter on September 11, 2023 at 5:01 pm Great question! We did put together a general post on source optimization to help folks get started on method development. There are suggested ranges for the various parameters in this post, for the various SCIEX sources. Performing ramps across the ranges and finding the optimal settings by monitoring peak intensity and spray stability is a great way to tune. Some parameters are related too, like GS2 and Temperature, and protrusion and GS1, so these are good to do in combination. General source/gas settings to use when starting method development Some compounds will of course fall outside these ranges if they are particularly fragile or hard to ionize. You can also reach out to our technical support team on the phone and they can help guide you with any tricky optimizations! Log in to Reply