Ever thought of breaking the high-throughput sound barrier for drug discovery?

Aug 7, 2019 | Blogs, Pharma | 0 comments

Wouldn’t it be great if we really could “get time back” or even “buy time”? When developing pharmaceuticals, it takes years to bring a new therapy to the market due to the linear nature of the process. As the saying goes, “Time waits for no one.” But what if we could do more in the same period, effectively slowing time down? Then we would be in the favorable situation of having time on our hands.

In pharmaceutical development, many new compounds are screened for their effectiveness during the discovery process. The efficacy is determined by various tests to ensure that the compounds are effective and safe. Many analytical technologies, all with different capabilities, are employed to screen the vast numbers of compounds and deliver an analytical determination. Let’s take high-throughput screening (HTS) assays in pharmaceutical development as an example. Have you ever considered that these are potential bottlenecks to sample throughput today? Have you ever thought that the effort it takes to develop and validate an assay is overly time-consuming? Or been concerned that the results from HTS assays have high numbers of false positives/negatives that mean you spend even more time on data evaluation? Then mass spectrometry (MS) based systems could be the way to improve the selectivity of results, gain confidence in the data, and provide the ability to multiplex—allowing you to do more on a single system. But MS-based technology has its pitfalls, too. One potential bottleneck, particularly with MS-based detection, is that it often requires the use of liquid chromatographic (LC) separation to help with the removal of chemical and matrix-related interferences. This enhances compound ionization and thus selective detection, but adds time to the analysis. Up until today, some of the quickest analysis times with MS have been in the region of 1 sample every 15 seconds.

So, have you heard about Acoustic Ejection Mass Spectrometry technology (AEMS) recently introduced at ASMS 2019 by SCIEX? This technology has the potential to surpass the limits of sample analysis throughput and revolutionize HTS in both speed, accuracy, and precision. With the Echo MS system, the speed of analysis can be as fast as 3 samples per second—50 times faster than current MS-based assays. Your current and future high-throughput screening workflows can be transformed with this new frontier in contactless sampling. The Echo MS system combines the pioneering innovations of an Open Port Interface (OPI) and Acoustic Droplet Ejection (ADE) to form an Acoustic Ejection Mass Spectrometry system. Powerful but gentle in its approach, ADE technology is built into a liquid handler. It focuses ultrasonic acoustic energy at the meniscus of a fluid sample to eject small droplets of liquid (between 1 and 10 nL) from microtiter plates wells (96, 384 or 1536) into the OPI. That is where the very accurate droplets are transferred to a SCIEX mass spectrometer ion source for detection using mass analysis. This results in answers at speeds of up to 180 samples/min, or about 260,000 per day. The accuracy and precision of the assays benefit from the capability of mass spectrometry analysis to deliver <5% CV, along with high levels of uptime because of the use of the OPI in combination with the proprietary SCIEX OptiFlow® Turbo V source. Other key benefits include:

  • Eliminating tip cross-contamination through tipless transfer
  • Providing unsurpassed accuracy and reproducibility of results
  • Preserving sample integrity and viability during transfer
  • Increasing flexibility in transferring liquids at different positions and volumes
  • Reducing operating costs by eliminating disposable tips and reducing waste generation

Not convinced? Learn More >

Register your interest in AEMS and gain insight into the potential of this new technology over the coming months.

Understanding PFAS and its impact on U.S. drinking water

In recent years, per- and polyfluoroalkyl substances (PFAS), often referred to as “forever chemicals,” have become a growing topic of interest due to their persistence in the environment and potential health risks. These synthetic compounds have been widely used in various industrial applications and consumer products since the 1940s. PFAS can be found in the air, soil, and water, and studies have shown that most people have detectable levels of PFAS in their bloodstream. One of the main exposure pathways for humans is through drinking water, particularly in communities located near industrial sites, military bases, or areas where firefighting foam has been used.

Selecting an LC-MS system for quantitation of pharmaceutical drug development

We understand you are busy, needing to prioritize running instruments, reporting results and managing your laboratory to meet deadlines. We created a solution guide to explain how SCIEX systems fit in the drug development pipeline to save you time evaluating options.

Nitrosamines: Where are we now?

Nitrosamines are a large group of N-nitroso compounds that share a common functional N-N=O group. They are produced by a chemical reaction between a nitrosating agent and a secondary or tertiary amine. Back in 2018, nitrosamines suddenly found themselves in the spotlight when they were unexpectedly detected in medications for high blood pressure. Since then, they have been found in several other prescription medications, including those for heartburn, acid reflux and diabetes, resulting in manufacturers recalling some common medications.

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