GEN-MKT-18-7897-A
Nov 18, 2020 | Blogs, Food / Beverage | 0 comments
Read time: 4 Minutes
The COVID-19 pandemic has increased the need to store and transport raw and finished food products in order to sustain the richness of the food supply chain. Therefore, having a robust post-harvest support system that monitors moisture levels and conditions of raw and finished food products is much needed to ensure food safety and security. I think this is a good time to talk about the risk of mycotoxin growth.
Mycotoxins are small, stable metabolites produced by molds and fungi that can contaminate a variety of crops. These naturally produced chemicals can provoke a toxic response when consumed even in low concentrations by both humans and animals.1
The thing to bear in mind is that toxin contamination usually stems from the source, with the 3 main contamination patterns being:
To put it into perspective, a toxin like aflatoxin is classified as a human carcinogen by the International Agency for Research on Cancer (IARC). These toxins typically make their way into our food chain through a pathway like this: ruminants consume feedstuff contaminated with aflatoxin B1 (AFB1), and the toxin metabolizes into aflatoxin M1 (AFM1). The AFM1 that gets excreted into milk is then consumed by humans, thus putting us at risk.
If you’re interested in learning more about the “what” and “how” of mycotoxin analysis, please check out this blog. In this post, I’d like to discuss a robust approach to screening for mycotoxins and their secondary metabolites, starting with shedding some light on masked mycotoxins.
Masked mycotoxinsCommonly studied mycotoxins include aflatoxins (e.g., AFB1), trichothecenes (e.g., DON), fumonisins (e.g., FB1), ochratoxins (OTA) and zearalenone (ZEN), and the 3 main genera of filamentous fungi that produce these toxins are aspergillus, fusarium and penicillium. Many food labs routinely test for mycotoxins, and research on the different types of mycotoxins is widely available and understood. On the flip side, however, there are potentially hundreds, maybe thousands, of other metabolites produced by plant defense mechanisms that can alter or degrade the mycotoxins of which we are aware. This can result in a slew of similar species present in food commodities that are structurally similar to the routinely targeted mycotoxins, but are not included in routine analytical panels. This means, from the perspective of a targeted analytical method, that they are present but unseen, and hence “masked,” and masked mycotoxins are not currently regulated by the same provisions that regulate routinely measured mycotoxins.
A masked mycotoxin can exhibit toxicity that is similar to its parent toxin. For example, the plant might modify the chemical structure of the toxin with a glucose or sulfate moiety, which reduces its toxicity to the plant. The plant itself may now contain only the conjugated form of the toxin, but during human or animal digestion, if the conjugate functional groups are degraded, the original mycotoxin may emerge and expose the consumer to the dangers of the toxin.2
Emerging awareness of the risks posed by these unseen toxins led some researchers to look beyond the targeted methods for the original compounds. Current knowledge of these “emerging mycotoxins” (e.g., NXToxins), as well as masked or other modified forms of mycotoxins, is limited, but the number of compounds that need to be analyzed is increasing rapidly, requiring more comprehensive analytical methods.3
Routine mycotoxin screeningThere is no true silver bullet for tackling the threat of mycotoxins and their metabolites. However, being able to evaluate their presence is key. There are several methods available for detection, all with advantages and limitations. One common technique—enzyme-linked immunosorbent assays (ELISA)—is rapid and easy, but it has poor selectivity sensitivity and specificity, which can be a problem, especially when detecting masked mycotoxins.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) can help address that gap. Our team at SCIEX has done some extensive work to put together a robust and high-throughput method to quantify 530 mycotoxins, masked mycotoxins and other metabolites.
This method was demonstrated on barley and corn extract using the SCIEX Triple Quad™ 5500+ LC-MS/MS System – QTRAP® Ready, and the results showed:
If you’re interested, you can click here to download the technical note that details this high-throughput mycotoxin analysis.
References
The ability to consistently achieve reproducible results on many complex samples across multiple days is critical to a routine clinical laboratory. Laboratories relying on analytical instrumentation require stability and robustness to perform a variety of screening and confirmatory assays with confidence. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become the preferred analytical method in the clinical laboratory to reliably perform clinical testing as it provides best-in-class performance and reliability for the most challenging assays. LC-MS/MS offers the required levels of sensitivity and specificity for the detection and quantitation of molecules from complex biological samples, helping laboratories deliver highly accurate data for a variety of clinically relevant analytes across a wide range of assays.
Depending on the samples that you are running on the system, it is possible for the Echo MS electrode to become dirty or occluded over time. Below are two different cleaning strategies that will be helpful for you to maintain your system and keep your electrodes running well.
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