How do I interpret the output from PCA? by Christie Hunter | Aug 6, 2021 | Data Visualization, MarkerView, SCIEX information, TechnologyPrincipal component analysis (PCA) is a data transformation technique that can be used to reduce the complexity of high-dimensional data sets (such as mass spectrometry data) while grouping samples based on common features. REMINDER! Principal components are linear...
What is principal component analysis and how does it work? by Christie Hunter | Aug 6, 2021 | Algorithms, Data processing, MarkerView, SCIEX information, TechnologyWhen we measure anything, the resulting measurements are often referred to as variables or dimensions. For example, a cube has three dimensions that can be measured: length, width and height. When we measure a complex sample using mass spectrometry (like a proteome,...
Peptide grouping in ProteinPilot software 5.0 by Christie Hunter | Aug 5, 2021 | Data Visualization, Life Science Research, ProteinPilot, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemA new feature in ProteinPilot software 5.0 is the ability to do peptide grouping. When analyzing proteomics data from LC-MS/MS, one must take into account the multiple pieces of evidence supporting a peptide as well as any ambiguity that might still exist in the...
How does ProteinPilot software obtain the annotations during a database search? by Alexandra Antonoplis | Aug 5, 2021 | Data Visualization, Life Science Research, ProteinPilot, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemWith the newest ProteinPilot software 5.0, we can annotate the database search results with ontology information about each protein using the information available from the UniProt website. After the final list of proteins is assembled during the search, the software...
Quickly compare identification results from ProteinPilot software by Christie Hunter | Aug 5, 2021 | Data Visualization, Life Science Research, ProteinPilot, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemWhen a ProteinPilot Software search is complete, a ProteinPilot report is generated that contains all the false discovery rate (FDR) analysis information. More information on using the large and small ProteinPilot reports can be found here When doing method...