What is principal component analysis and how does it work?
When we measure anything, the resulting measurements are often referred to as variables or dimensions. For example, a cube has three dimensions that can be measured: length, width and height. When we measure a complex sample using mass spectrometry (like a proteome,...
Peptide grouping in ProteinPilot software 5.0
A new feature in ProteinPilot software 5.0 is the ability to do peptide grouping. When analyzing proteomics data from LC-MS/MS, one must take into account the multiple pieces of evidence supporting a peptide as well as any ambiguity that might still exist in the...
How does ProteinPilot software obtain the annotations during a database search?
With the newest ProteinPilot software 5.0, we can annotate the database search results with ontology information about each protein using the information available from the UniProt website. After the final list of proteins is assembled during the search, the software...