Using ProteoWizard for converting files – for use in ProteinPilot software by Jenny Albanese | Aug 5, 2021 | Data processing, Life Science Research, ProteinPilot, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemProteoWizard is a set of open-source tools and libraries for proteomics researchers and includes file conversion tools that allow vendor-specific file formats to be converted to standard formats (mgf, mzML, mzXML and mzData). Learn how to download and install...
CloudTalk – CloudConnect and Experiment Manager for metabolomics by Alexandra Antonoplis | Aug 5, 2021 | Getting Started, How-to videos, Life Science Research, Metabolomics, OneOmicsThis Cloud Talk (7:44 min) describes how to upload data files to the SCIEX Cloud and organize them in Experiment Manager for metabolomics SWATH acquisition data processing
Computing protein confidence with improved accuracy by reassessing peptide confidence during protein grouping by Christie Hunter | Aug 5, 2021 | Data processing, Life Science Research, ProteinPilot, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemProteinPilot software 4.0 and higher releases include a new method for calculating protein confidences that improves reliability at the end of protein lists. Figure 1 shows a simulated example that demonstrates how more accurate protein confidence is computed. Figure...
Unused Prot Score in ProteinPilot software by Christie Hunter | Aug 5, 2021 | Data processing, Life Science Research, ProteinPilot, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemThere are 2 key protein scores that are computed in ProteinPilot™ Software: Total ProtScore and Unused ProtScore. Total ProtScore is simply a sum of all the peptide evidence related to a given protein, while Unused ProtScore reflects the amount of total, unique...
How does multi-precursor searching work in ProteinPilot software 5.0? by Christie Hunter | Aug 5, 2021 | Data processing, Life Science Research, ProteinPilot, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemIn highly complex proteomics data, some percentage of MS/MS spectra is chimeric due to the transmission of more than 1 precursor through the MS isolation step, even with a 1 Da window like the one used on TripleTOF systems. The ProteinPilot software 5.0 release...