What is the Most likely ratio normalization strategy? by Christie Hunter | Aug 7, 2021 | Algorithms, Life Science Research, MarkerView, Metabolomics, OneOmics, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemWhen performing LC-MS quantitation, there are numerous sources of experimental variance that can confound the quality of your results (variation in the starting amount of sample, variation in the LC-MS measurements, etc.). Having a robust normalization strategy that...
What is the difference between the gobal and the local FDR rates in ProteinPilot software? by Christie Hunter | Aug 6, 2021 | Data processing, Life Science Research, ProteinPilot, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemThe main idea of the target-decoy approach to compute false discovery rates is to feed the search engine answers that you know are wrong. These are referred to as ‘decoys’, typically generated by reversing, randomizing, or scrambling real protein sequences. You then...
Protein alignment template for results comparisons across multiple group files from ProteinPilot software database searches by Christie Hunter | Aug 6, 2021 | Data Visualization, Life Science Research, ProteinPilot, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemThe key challenge in aligning protein level results is flexible matching by making use of the accession ambiguity in reported protein groups. The better this task is done, the more complete will be the protein matching across multiple results. For quantitative work, a...
How do I use the new ProteinPilot software reports (small vs large)? by Alexandra Antonoplis | Aug 6, 2021 | Data Visualization, Life Science Research, ProteinPilot, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemAt the end of a database search, the software will write information into the ProteinPilot Report automatically. The ProteinPilotReport.xlsx file must be placed in the following directory for the automatic creation to work. C:\Program Files\AB...
Downloading and viewing ProteinPilot app group files on your desktop and extracting key exports by Alexandra Antonoplis | Aug 6, 2021 | Data processing, Life Science Research, OneOmics, ProteinPilot, Proteomics, SCIEX informationAfter the database search in OneOmics suite, the ProteinPilot software group file can be downloaded for viewing in your desktop ProteinPilot software 5.0.3. Please note that this is a beta version of ProteinPilot software, and as such is unsupported and provided...