How can I tell if my Spectral Peak is saturated on the TripleTOF system? by Christie Hunter | Aug 8, 2021 | Data acquisition, QTOF, SCIEX information, TechnologyThe key performance attributes for qualitative analysis are speed, mass accuracy, and resolution in both MS and MS/MS modes. For quantitative analysis, inter-scan dynamic range is important in order to maintain accuracy and linear response over a wide range of sample...
How does Dynamic Ion Transmission Control (DITC) work on the TripleTOF systems? by Christie Hunter | Aug 8, 2021 | Data acquisition, QTOF, SCIEX information, Technology, ZenoTOF 7600 systemDynamic Ion Transmission Control (ITC) is the feature on all the QTOF systems from SCIEX where the MS ion current through the instrument is modulated scan by scan to ensure the detector saturation point is not reached. The modulation is done both at the level of total...
What is the difference between MRM3 vs MS/MS/MS (MS3)? by Christie Hunter | Aug 8, 2021 | Data acquisition, QTRAP / Triple Quad, SCIEX information, TechnologyThe MRM3 workflow and the MS3 scan are functionally the same QTRAP system scan, but used with different goals in mind. The main difference is how these scans are used in the whole MS workflow. With MS3 scans, you can use these in a data dependent mode for discovery...
Protocol for offline trap washing with NanoLC 425 system by Christie Hunter | Aug 7, 2021 | Data acquisition, LC, Life Science Research, Proteomics, SCIEX informationUsing the dual gradient system, the nanoLC 425 system can be configured such that the trap can be washed to waste rather than through the column and into the mass spectrometer. This workflow can be performed with either nanoflow or microflow chromatography. Figure 1....
Analysis of chiral amphetamines using differential mobility separation (DMS) by Pierre Negri | Aug 7, 2021 | Data acquisition, Differential Mobility Separation (DMS), SCIEX information, TechnologyMany compounds are challenging to analyze because they are isomers (have the same m/z) and also sometimes have the same HPLC retention time. And to make it even more complicated, sometimes there are two chiral forms that have different functions in biology, making it...