Han Wang

Journal publications using the Echo® MS (+) system

Jun 17, 2025 | Blogs, Echo® MS+ system, Pharma | 0 comments

The Echo^® MS+ system is a novel platform for Acoustic Ejection Mass Spectrometry (AEMS) and combines the speed of acoustic sampling with the selectivity of mass spectrometry. This platform has been designed for high throughput analysis of small and large molecules. The technology combines Acoustic Droplet Ejection (ADE), an Open Port Interface (OPI) and could be coupled with the SCIEX Triple Quad 6500+ system or the ZenoTOF 7600 system. Learn more about the platform here.

Check out the following applications using the Echo^® MS+ system below:

Review Publications

Shou, W.Z.; Acoustic ejection mass spectrometry: Development, applications, and future perspective. Biomedical Chromatography, 2022 Feb;36(2):e5278.
Liu, C.; Acoustic ejection mass spectrometry: fundamentals and applications in high-throughput drug discovery. Expert Opinion on Drug Discovery, 2022 Jul;17(7):775-787.

Liu, C.; High-throughput MS for intact protein analysis. Bioanalysis, 2023 Aug;15(16):1017-1019.

Liu, C.; Wen, X.; Mass Spectrometry in covalent drug discovery. Current Molecular Pharmacology, 2024;17(1):e18761429319065.

Liu, C.; Zhang, H.; Data processing for high-throughput mass spectrometry in drug discovery. Expert Opinion on Drug Discovery, 2024 19(7), 815–825.
Wen, X.; McLaren, D. G.; High-throughput hit identification with acoustic ejection mass spectrometry. SLAS Technology, 2025, Volume 31, 100245, ISSN 2472-6303.
Liu, C., Acoustic ejection Mass spectrometry: the potential for personalized medicine. Expert Review of Proteomics, 2025 Apr;22(4):141-147

Research System Publications

Echo^® MS+ system technology

Häbe, T. T.; Liu. C; Covey, T.M., al. Ultrahigh-throughput ESI-MS: Sampling pushed to six samples per second by acoustic ejection mass spectrometry. Analytical Chemistry 2020, 92 (18), 12242-12249. (also discusses HT-ADME and Bioanalysis)

Liu, L.; Van Berkel, G.J.; Cox, D. M.; Covey T. R., Operational modes and speed considerations of an acoustic droplet dispenser for mass spectrometry. Analytical Chemistry 2020, 92 (24), 15818-15826.
Guo, Y.; Forbush, M.; Covey, T.R.; Ghislain, L.; Liu, C.; High-throughput analysis from complex matrices: Acoustic ejection mass spectrometry from phase-separated fluid samples. Metabolites, 2021 Nov;11(11):789-799. (also discusses Bioanalysis and Drug of Abuse)
Liu, C.; Van Berkel, G.J.; Kovarik, P.; Perot, J. B.; al. Fluid dynamics of the open port interface for high-speed nanoliter volume sampling mass spectrometry. Analytical Chemistry, 2021 93 (24), 8559-8567.

Zhang, H.; Liu, C.; Hua, W.; Ghislain, L. P.; al. Acoustic ejection mass spectrometry for high-throughput analysis. Analytical Chemistry, 2021 93 (31), 10850-10861. (also discusses HT-ADME, HTS, HTE and Bioanalysis)

Ma, J.; Aw, C. C.; Ji, H.; Lin, S.; Yin, X.; Tey, H.; Liu, C.; High-throughput acoustic ejection mass spectrometry with adjustable signal durations. Analytical Chemistry, 2024 96 (14), 5357-5362.

Cox, D. M.; Yin, X.; Alam, J.; Georgescu, B.; al. Sample-specific MS/MS methods in high-throughput mass spectrometry. Journal of the American Society for Mass Spectrometry, 2024 35 (9), 2230-2236.

Ji, H.; Yin, X.; Ang, W. E.; Rawshan, A. B.; al. Automatic cleaning in acoustic ejection mass spectrometry: Enhancing the system robustness for large-scale high-throughput analysis of complex samples. SLAS Technology, 2024, Volume 29, Issue 6, 100227, ISSN 2472-6303.

Verma, M.; Hoxie, N.; Janiszewski, J.; Bonney, C.; Hall, M. D.; Michael, S.; Covey, T.; Shrimp, J. H., Notes on AEMS Methods Development for High Throughput Experimentation in Drug Discovery. SLAS Technology 2024, 29 (6), 100234, ISSN 2472-6303

High Throughput Screening (HTS)

Wen, X.; Liu, L.; Ghislain, L.; Tovar, K.; Shah, V., al. Direct analysis from phase-separated liquid samples using ADE-OPI-MS: Applicability to high-throughput screening for inhibitors of diacylglycerol acyltransferase 2. Analytical Chemistry 2021, 93 (15), 6071-6079.

Winter, M.; Simon, R. P.; Häbe, T. T.; Ries, R.; Wang, Y.; Kvaskoff, D.; Fernández-Montalván, A.; Luippold, A. H.; Büttner, F. H.; Reindl. W.; Label-free high-throughput screening via acoustic ejection mass spectrometry put into practice. SLAS Discovery, 2023 Jul;28(5):240-246.

Bazargan, S.; Dranchak, P.; Liu, C.; Inglese, J.; Janiszewski, J.; Schneider, B. B.; Covey, T. R., Differential Mobility Spectrometry Acoustic Ejection Mass Spectrometer System for Screening Isomerization-Mediating Enzyme Drug Targets. Analytical Chemistry, 2024, 96 (52), 20645-20655.

High Throughput Experimentation (HTE)

DiRico, K. J.; Hua, W.; Liu C, al. Ultra-high-throughput acoustic droplet ejection-open port interface-mass spectrometry for parallel medicinal chemistry. ACS Medicinal Chemistry Letters 2020, May 1;11(6):1101-1110.

High Throughput (HT) ADME

Hollenbeck, T.; Thibodeaux, S.; White, P.; Westling, L.; Chong, A.; Isbell, J.,
Acoustic droplet ejection and open port interface for rapid analysis of metabolic stability assays. Journal of Pharmaceutical Sciences 2020, Nov; 109(11):3285-3291.
Wagner, A.; Zhang, J.; Liu, C.; Covey, T.M., al. Ultrahigh-throughput and chromatography-free bioanalysis of polar analytes with acoustic ejection mass spectrometry. Analytical Chemistry 2020, 92 (19), 13525-13531.
Rimmer, M. A.; Twarog, N. R.; Li, Y.; Shelat, A. A.; al. A high-throughput quality control method for assessing the serial dilution performance of dose–response plates with acoustic ejection mass spectrometry. SLAS Technology, 2024, Volume 29, Issue 1, 100115, ISSN 2472-6303.

Compound Quality Assessment (QA)

Zhang, J.; Zhang, Y.; Liu, L.; Covey T. R., al. Acoustic ejection/full-scan mass spectrometry analysis for high-throughput compound quality control.
SLAS Technology, Volume 26, Issue 2, 2021, Pages 178-188, ISSN 2472-6303.

Zhang, J.; Liu, C.; Veiga, C.; Covey, T.R.; Shou, W.; Weller, H.;
A full scan data review tool to match the speed of acoustic ejection mass spectrometry, LCGC North America, 2022 40(7): p. 314–320.

Quinn, A.; Ivosev, G.; Chin, J.; Mongillo, R.; Veiga, C.; Covey, T. R.; al.
High-throughput compound quality assessment with high-mass-resolution acoustic ejection mass spectrometry: An automatic data processing toolkit. Analytical Chemistry, 2024 96 (21), 8381-8389.

Hoxie, N.; Calabrese, D. R.; Itkin, Z.; Gomba, G.; al. High-resolution acoustic ejection mass spectrometry for high-throughput library screening. SLAS Technology, 2024, Volume 29, Issue 6, 100199, ISSN 2472-6303.

Intact Biologic Analysis

Zacharias, A. O.; Liu, C.; VanAernum, Z. L.; Covey, T.R.; Ultrahigh-throughput intact protein analysis with acoustic ejection mass spectrometry. Journal of the American Society for Mass Spectrometry, 2023 34 (1), 4-9.

Covalent Binding

Wen, X.; Liu, C.; Tovar, K.; Curran, P.; Richards, M.; al. High-throughput covalent modifier screening with acoustic ejection mass spectrometry. Journal of the American Chemical Society, 2024 146 (29), 19792-19799.

Synthetic Biology

Holland-Moritz, D.; Moor, S.; Parry, J.; Medcalf, E.; Eberle, C.; Strakham, A.; Grosser, S.; Hu, H.; Dunham, N.; Gantz, M., Accelerating Cross-Modality Reaction Optimization via Robotically Automated Vacuum Enabled Direct-inject Mass Spectrometry (RAVE MS). 2025, DOI:10.26434/chemrxiv-2025-g242r.

Commercial System Publications

High Throughput Screening (HTS)

Simon, R.P.; Häbe, T.T.; Ries, R.; Winter, M.; Wang, Y.; al. Acoustic ejection mass spectrometry: A fully automatable technology for high-throughput screening in drug discovery. SLAS Discovery: Advancing the Science of Drug Discovery, 2021;26(8):961-973.

Hasegawa, T.; Imamura, R.M.; Suzuki, T.; Hashiguchi, T.; Nomura, T.; Otsuguro, S.; al. Application of acoustic ejection MS system to high-throughput screening for SARS-CoV-2 3CL protease inhibitors. Chemical and Pharmaceutical Bulletin (Tokyo), 2022 Mar 1;70(3):199-201.

Speckmeier, E.; Pommereau, A.; Grosser, K.C.; Mors, H.; al. A high-throughput screening assay for mutant isocitrate dehydrogenase 1 using acoustic droplet ejection mass spectrometry. SLAS Discovery, 2022 Jul;27(5):298-305.

Silhan, J.; Klima, M.; Otava, T.; Skvara, P.; Chalupska, D.; Chalupsky, K.; al. Discovery and structural characterization of monkeypox virus methyltransferase VP39 inhibitors reveal similarities to SARS-CoV-2 nsp14 methyltransferase. Nature Communications, 2023 Apr 20;14(1):2259.

Štefek, M.; Chalupská, D.; Chalupský, K.; Zgarbová, M.; al. Rational design of highly potent SARS-CoV-2 nsp14 methyltransferase inhibitors. ACS Omega, 2023 Jul 21;8(30):27410-27418.

Krejčová, K.; Krafcikova, P.; Klima, M.; Chalupska, D.; Chalupsky, K.; al. Structural and functional insights in flavivirus NS5 proteins gained by the structure of Ntaya virus polymerase and methyltransferase. Structure, 2024 Aug 8;32(8):1099-1109.e3.

Bärenz, F.; Zuschlag, Y.; Pommereau, A.; Warkentin, J.; Licher, D. T., The development of a high-throughput acoustic droplet ejection mass spectrometry assay and a solid-supported membrane (SSM)-based electrophysiological assay to study the pharmacological inhibition of SLC1-A3,-A2 and-A1 in a drug discovery program. Frontiers in Pharmacology 2025, 16, 1544682.

High Throughput Experimentation (HTE)

Hu, M., Yang, L., Twarog, N. et al. Continuous collective analysis of chemical reactions. Nature, 2024, 636, 374–379.

High Throughput (HT) ADME

Břehová, P.; Řezníčková, E.; Škach, K.; Jorda, R.; al. Inhibition of FLT3-ITD kinase in acute myeloid leukemia by new imidazo[1,2‑b]pyridazine derivatives identified by scaffold hopping. Journal of Medicinal Chemistry, 2023 66 (16), 11133-11157.

Skácel, J.; Djukic, S.; Baszczyňski, O.; Kalčic, F.; Bílek, T.; Chalupský, K.; al. Design, synthesis, biological evaluation, and crystallographic study of novel purine nucleoside phosphorylase inhibitors. Journal of Medicinal Chemistry, 2023 66 (10), 6652-6681.

Covalent Binding

Stoeckli, M.; al. Accelerating covalent binding studies: Direct mass shift measurement with acoustic ejection and TOF-MS. SLAS Technology, 2024, Volume 29, Issue 6, 100216, ISSN 2472-6303.

Cheng, R., Muneer, A., Hercher, M., Bajrami, B., & Nemati, R., Acoustic ejection mass spectrometry: An integrated pipeline for ultra-high throughput screening, reactivity profiling, and potency analysis of covalent BTK inhibitors. SLAS Technology, 2025, Volume 33, 100307, ISSN 2472-6303.

Synthetic Biology

Zhang, Z.; Zhang, C.; Zhang, X.; Chen, J.; al. Automated and integrated ultrahigh throughput industrial strain screening enabled by acoustic-droplet-ejection mass spectrometry. Journal of Pharmaceutical Analysis, 2024, Volume 14, Issue 9, 100949, ISSN 2095-1779.
Hu, H.; Singh, A. N.; Lehnherr, D.; Mdluli, V.; al. Accelerating pharmaceutical process development with an acoustic droplet ejection-multiple reaction monitoring-mass spectrometry workflow. Analytical Chemistry, 2024 96 (3), 1138-1146.
Hu, L.; Zhang, Z.; Li, C.; Han, M.; al. High-throughput screening for oligonucleotide detection by ADE-OPI-MS. Analytical Chemistry, 2024 96 (29), 12040-12048.

Li, Z.; Li, Z.; Xu, Q.; Zhao, L.; Li, B.; Liu, W.; Shi, Y.; Acoustic ejection tandem mass spectrometry for high-throughput screening of phencyclidine-type substances in urine, including authentic cases. Analytica Chimica Acta, 2024, Volume 1312, 342751, ISSN 0003-2670.

Additional Publications:

Drouin, N.; Liu, C.; Cox, D.M.; Young, J. B.; al. High throughput mass spectrometry assay for early chick gender determination: less than 3s total analysis time per sample. ChemRxiv, 2023; doi:10.26434/chemrxiv-2023-8z9gp. (Egg Gender Determination)

Yang, F.; Ma, K.; Cao, Y.; Li, Z.; Application of magnetic materials combined with Echo® mass spectrometry system in analysis of illegal drugs in sewage. 2024 Apr 29;29(9):2060. (Drug of Abuse)

Van Puyvelde, B., Hunter, C.L., Zhgamadze, M. al. Acoustic ejection mass spectrometry empowers ultra-fast protein biomarker quantification. Nature Communications, 2024, 15, 5114. (Peptide Quantification)
Na An, Peng-Cheng Mei, Ao Li, Xiu-Feng Fu, Xin-Ze Wu, Han-Peng Jiang, Quan-Fei Zhu, and Yu-Qi Feng.; al. Derivatization-Assisted Acoustic Ejection Mass Spectrometry for High-Throughput FAHFA Prescreening. Analytical Chemistry 2025 97 (18), 9613-9619. (Lipid Analysis)
Rimmer, M. A., Twarog, N., Ranathunge, T., Wang, J., Li, Y., Chen, T., Shelat, A., & Yang, L. (2025). rtmsEcho: An Open-Source R Package for Automated Analysis of Acoustic Ejection Mass Spectrometry Data. bioRxiv. 2025. (Data Processing)

Getting PFAS right: Why sample preparation matters

Ultra‑low reporting limits, expanding target lists, and the constant risk of background contamination mean that even small missteps before injection can compromise data integrity. PFAS can be introduced at nearly every stage of prep, from sampling containers and PPE to SPE cartridges, filters, solvents, and lab consumables, making contamination control as critical as analyte recovery.

CE‑SDS in monoclonal antibody therapeutic development

In monoclonal antibody (mAb) development, assessment of purity and integrity of the protein in question is critical. CE‑SDS is the gold standard assay and is routinely run from analytical development through QC and lot release. It’s trusted because it consistently delivers quantitative, size‑based insight into purity and fragmentation, and it fits naturally into regulated environments.

EAD vs. ETD: Choosing the right fragmentation technique for small molecule Met ID studies

In drug discovery and development, Metabolite Identification (Met ID) plays a critical role in understanding biotransformation pathways, ensuring safety, and meeting regulatory requirements. Advanced mass spectrometry techniques have revolutionized this process, particularly through electron-based fragmentation methods such as Electron Activated Dissociation (EAD) and Electron Transfer Dissociation (ETD). While both techniques leverage electron interactions to generate informative fragment ions, they differ significantly in mechanism, performance, and suitability for Met ID workflows.

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Han Wang

Dr. Wang Han is the Senior Technical Product Manager, Hyphenation at SCIEX, based in Singapore. During the past 4years, her role has been pivotal in the successful launch of Echo MS+, improving commercial products, and evaluating new technologies to stay ahead in the competitive landscape. Han has consistently ensured that new products meet customer needs by accurately translating and continually validating system requirements throughout the development process. Her meticulous approach is further exemplified through the execution of the Early Customer Evaluation (ECE) program and her active contribution to customer feedback loops. Han’s specialization lies in Acoustic Ejection Mass Spectrometry (AEMS), chromatography and MS. Her expertise encompasses a deep understanding of customer applications and workflows across various markets, including Biopharma, Pharma, and Omics. This extensive knowledge allows her to tailor solutions that meet the specific needs of diverse industries, driving advancements and enhancing productivity. Prior to joining SCIEX, Han worked at Waters Singapore, where she led a team of five Applications Consultants to drive business growth in Chromatography and MS while providing technical support to customers across Southeast Asia (SEA).

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