GEN-MKT-18-7897-A
Apr 28, 2026 | Biopharma, BioPhase 8800 system, Blogs | 0 comments
CE‑SDS remains a cornerstone assay for characterizing fragmentation, aggregation, and product‑related impurities in therapeutic proteins. UV detection has been the long‑standing standard. However, it frequently struggles with baseline noise, limited sensitivity for minor fragments, and subjective integration.
Native fluorescence detection (NFD) strengthens CE‑SDS performance by improving both detection sensitivity and interpretability.
High sensitivity without labeling
NFD leverages intrinsic tryptophan fluorescence to detect proteins label‑free, avoiding the variability and workflow burden of dye‑labeling steps.
Technical note data show:
For scientists managing stability programs or performing forced degradation studies, this sensitivity can reveal early‑stage degradation signatures that UV cannot reliably resolve.
Clearer, more stable baselines for better integration
Typical UV electropherograms show baseline drifting due to buffer absorbance and gel heterogeneity.
The NFD baseline, however, is consistently flat, improving:
These benefits reduce manual reprocessing and strengthen data defensibility across development stages.
Multi-capillary throughput for faster studies
The BioPhase 8800 system processes 8 samples in parallel, expanding CE‑SDS throughput without requiring additional instruments or longer sequences.
High‑throughput CE‑SDS paired with NFD’s sensitivity provides a strong foundation for data‑driven therapeutic development.
A balanced upgrade path from UV and LIF
NFD complements existing detection modes:
This offers scientists flexibility while building long‑term method robustness.
Explore the CE‑SDS NFD data
For deeper review, check out the resources in our NFD solutions hub >
At SCIEX, innovation doesn’t stop at instruments; it extends to how you interact with your LC-MS/MS or CE systems every day. That’s why we’re excited to introduce the SCIEX Now spring 2026 improvements: a set of meaningful enhancements shaped directly by your feedback.
In today’s fast-paced analytical laboratories, uptime is not just important; it is mission-critical. Whether you are running high-throughput bioanalysis, environmental testing, or pharmaceutical workflows, every minute of LC-MS downtime can impact productivity, data quality, and timelines.
That is why we are excited to introduce a smarter and faster way to troubleshoot.
Ultra‑low reporting limits, expanding target lists, and the constant risk of background contamination mean that even small missteps before injection can compromise data integrity. PFAS can be introduced at nearly every stage of prep, from sampling containers and PPE to SPE cartridges, filters, solvents, and lab consumables, making contamination control as critical as analyte recovery.
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