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Standard Front-End Cleaning for TripleTOF systems

christie.hunter@sciex.com
Member Admin

General points:

  • Use of laboratory deionized (DI) water is specified at points in the procedure.  However, if there are any concerns regarding quality, please utilize a reputable supplier of pure deionized water (>18.2M ohm).  5-10 liters will typically be required to perform cleaning and rinsing steps. 
  • If there are any concerns regarding tap water quality (color, clarity, residue etc), utilize pure DI water instead, as specified above.
  • Always change gloves post detergent cleaning & prior to final water rinsing/soaking steps.

(1)  Prepare SCIEX Cleaning Solution

  • Empty entire 15g packet of SCIEX Concentrated Powder Instrument Cleaner (P/N 5042862) into a Nalgene bottle (see parts list on page 3). 
  • Fill with 1.5L of DI water (cold or room temperature).
  • Mix by shaking until all particles have dissolved.  This batch will last 8 hours max.

(2)  Clean Curtain Plate (CP)

  • Take lint free wipe and soak in cleaning solution. 
  • Use the wipe on both sides of the CP for 30 secs before discarding the wipe. 
  • Rinse CP with tap water for 2 mins. 
  • Repeat process a further x2 times. 
  • Perform a final DI water rinse for 30 secs. 
  • Immediately dry with a flowing N2 line until no visible droplets remain.
  • Temporarily store CP on food grade aluminum foil.

(3)  Clean Orifice Plate (OP)

  • Take lint free wipe and soak in cleaning solution. 
  • Use on both sides of the OP metal cone area for 30 secs before discarding the wipe (Figure 1).
  • Rinse OP with tap water for 2 mins. 
  • Repeat process a further x2 times. 
  • Perform a final DI water rinse for 30 secs.
  • Immediately dry with a flowing N2 line until no visible droplets remain.
  • Temporarily store on food grade aluminum foil.
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(4)  Clean QJet® Ion Guide (QJ) 

  • Remove IQ0 lens and set aside.
  • Take large brush and rinse with tap water.  Immerse the brush in cleaning detergent.
  • Scrub ALL accessible QJ surfaces including rod set ends for a minimum of 2 mins (Figure 2).
  • Rinse QJ with tap water for 2 mins. 
  • Take medium brush and rinse with tap water.  Immerse the brush into cleaning detergent. 
  • Scrub all accessible internal QJ surfaces (inner and outer rod surfaces) for a minimum of 2 mins (Figure 3).
  • Rinse QJ with tap water for 2 mins.
  • Repeat above process one further time.
  • Take a clean beaker or SCIEX silver flexible cleaning bag and fill with cleaning solution taken from Nalgene bottle.
  • Place QJ in beaker/bag and sonicate for 30 mins at room temperature.
  • Rinse QJ with tap water for 2 mins. 
  • Rinse QJ with DI water for 30 secs.
  • Take a clean beaker or SCIEX clear flexible cleaning bag and fill with DI water.
  • Place QJ in beaker/bag and sonicate for 30 mins at room temperature.
  • Dry QJ with a flowing N2 line until no visible droplets remain.
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(5)  Clean IQ0

  • Take large brush and rinse with tap water.  Immerse brush into cleaning detergent. 
  • Scrub IQ0 surfaces for 2 mins, attention made not to scratch lens with brush metal stem (Figure 4).
  • Rinse IQ0 with tap water for 2 mins.
  • Take medium brush and rinse with tap water.  Immerse the brush in cleaning detergent.
  • Scrub outer cone area (QJ facing) of IQ0 passing brush through cut-out gaps for a minimum of 2 mins (Figure 5).
  • Rinse IQ0 with tap water for 2 mins
  • Take lint free wipe and soak in cleaning solution. 
  • Use to clean the inner IQ0 cone area (Q0 facing) and discard the wipe.
  • Rinse IQ0 with tap water for 2 mins
  • Take a clean beaker or SCIEX silver flexible cleaning bag and fill with cleaning solution from Nalgene bottle.
  • Place IQ0 in beaker/bag and sonicate for 30 mins at room temperature.
  • Rinse IQ0 with tap water for 2 mins. 
  • Rinse IQ0 with DI water for 30 secs.
  • Take a clean beaker or SCIEX clear flexible cleaning bag and fill with DI water.
  • Place IQ0 in beaker/bag and sonicate for 30 mins at room temperature.
  • Dry IQ0 with a flowing N2 line until no visible droplets remain.
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Consumable Parts:

  • 5042862 - SCIEX Concentrated Powder Instrument Cleaner, 1 box of 50 packets
  • 5045687 - Powder-free neoprene gloves, 1 box of 100, large
  • 5042975 - 400 mL beaker, RED-coded, for washing
  • 5042976 - 400 mL beaker, WHILE-coded, for rinsing
  • 5021295 - Large cleaning brush
  • 5049373 - Medium cleaning brush
  • 5049475 - Small cleaning brush
  • 5047497 - Flexible Zip Bag - Silver, to wash large parts
  • 5047496 - Flexible Zip Bag - Clear, to rinse large parts
  • 5047495 - Flexible Nalgene mixing bottle, to make cleaning solution

 

RUO-MKT-18-8256-A

 

 
This topic was modified 7 months ago by christie.hunter@sciex.com
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Posted : August 14, 2018 4:15 pm
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