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Jun 27, 2019 | Blogs, Technology | 0 comments
Choosing the best technique for your analysis can be tough. Should you go with gas chromatography/mass spectrometry (GC-MS) or liquid chromatography/tandem mass spectrometry (LC-MS/MS)? That’s the key question. That’s why we’re here to help.
The Limitations of GC-MSGC-MS is an established technique to analyze volatile organic compounds (VOCs) and trihalomethanes (THMs). Simply put, it’s a technique that’s best used for thermally stable molecules. Traditionally, GC-MS is the preferred technique for the analysis of less polar and more volatile compounds such as organochlorine pesticides, dioxins, and polychlorinated biphenyl.
While the technique can offer good separation, the high temperatures used for vaporization during GC analysis can alter or degrade half of the analytes in a sample1. This is the primary concern for life science researchers who deal with relatively labile biological molecules that break down easily at high temperatures. GC-MS also becomes a challenge because it can involve labor-intensive sample preparation and long chromatographic run times.
The LC-MS/MS AdvantageLC-MS/MS combines the separation power of liquid chromatography with the identification and quantification power of tandem mass spectrometry.
The strength of this technique is in the ability of LC to separate a wide range of compounds before the tandem MS quantifies them with a high degree of sensitivity and selectivity based on the unique mass/charge (m/z) transitions of each compound of interest.
LC-MS/MS systems offer these basic benefits:
Many scientists who need increased levels of sensitivity, reproducibility, and robustness over GC-MS are either considering or have already made the leap to LC-MS/MS. When will you?
Let SCIEX Help You Make the Leap from GC-MS to LC-MS/MS
If your budget is holding you back, we have news for you. The SCIEX Triple Quad™ 3500 System provides all the advantages of modern LC-MS/MS technology. It’s the ideal choice for labs operating on a tight budget, and its simplicity of operation has the potential to expand your operation beyond the restrictions of gas chromatography.
Simply put, the Triple Quad 3500 LC-MS/MS System explained in fewer than 140 characters: Legendary power, speed, and accuracy are more affordable than ever.
Download your copy of the compendium to see what the SCIEX Triple Quad 3500 LC-MS/MS system can do for your laboratory.
Reference:
PFAS analysis is complex, but expert guidance doesn’t have to be. In this episode of our ‘Ask the PFAS expert series’, we’re joined by Michael Scherer, Application Lead for Food and Environmental, to answer the most pressing questions in PFAS analysis. From why LC-MS/MS systems are the gold standard for analyzing diverse PFAS compounds, to which EU methods deliver reliable results for drinking water, and to practical steps to prevent contamination, Michael shares actionable insights to help laboratories achieve accuracy, consistency, and confidence in their workflows.
During an LC-MS/MS experiment, traditional fragmentation techniques like collision-induced dissociation (CID) have long been the gold standard. Electron-activated dissociation (EAD) is emerging as a transformative tool that enhances structural elucidation, particularly for complex or labile metabolites.
In the field of food chemistry and health, Prof. Nils Helge Schebb and his team at the University of Wuppertal are at the forefront of applying cutting-edge analytical methods to investigate how dietary components affect inflammation and chronic disease. Their work focuses on lipid mediators, particularly oxylipins, and how these molecules can be precisely measured and interpreted using liquid chromatography-tandem mass spectrometry (LC-MS).
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