GEN-MKT-18-7897-A
Nov 7, 2019 | Blogs, Technology | 0 comments
Would you like to take your LC-MS/MS analysis from 0 to 3 mL/min without the complexity of changing probes or splitting the LC flow? Then take a look into the Turbo V ion source from SCIEX.
Introduced more than 15 years ago, the Turbo V ion source continues to be a gold standard for rugged and user-friendly source design, for both ESI and APCI ionization. This shrewd architecture provides outstanding robustness and sensitivity when analyzing complex biological matrices with minimal maintenance. By merging two orthogonal streams of hot gas in the ESI region, efficient desolvation and hydrodynamic focusing of ions towards the sampling orifice is achieved.
For higher flow rate applications, this can translate to boosts in sensitivity of several orders of magnitude over unheated ESI sources. Desolvation is greatly improved without disrupting the curtain gas flow and greatly boosts the robustness of the entire system. Figure 1 schematically represents the heated gas flows within the Turbo V source.
The success of the Turbo V ion source over the years served as the inspiration and foundation upon which the more recently introduced IonDrive Turbo V and OptiFlow Turbo V ion sources are designed. Each of these sources benefits from the use of orthogonally placed jets of heated gas directed at the liquid sample spray to enable even more efficiency of desolvation at different liquid flow regimes. While robustness and ruggedness were built in from the start, plug-and-play simplicity and a “just put it on, and it works” engineering is typical of all present-day SCIEX sources based on the Turbo V. Figure 2 highlights the wider heaters involved with the IonDrive Turbo V design for increased desolvation and thus higher sensitivity. The red square indicates the ‘sweet spot’ of standard Turbo V and the blue square highlights the bigger ‘sweet spot’ of IonDrive.
Key benefits of the IonDrive Turbo V Source
The recent introduction of OptiFlow technology allows the use of the Turbo V design to be translated to lower flow rate chromatography, such as nano and micro-flow rates. The benefit of lower flows can help with gains in sensitivity especially when you are ‘sample limited’. Significant research was done to develop a low-flow source that is sensitive while maintaining high robustness and ease of use of higher-flow sources. The result is an ion source, based on the legendary Turbo V design, with the ability to switch easily between nano and microflow regimes. No need to change the source, break vacuum and the system can be back up and running in minutes. Simple, robust and reliable. Figure 3 shows a picture of the Optiflow ion source based on the Turbo V geometry. Learn more about the legendary Turbo V ion source >
To see more about the 50 years of SCIEX mass spectrometry technology innovations visit the Generation Quant docu-series.
The Echo® MS+ system is a novel platform for Acoustic Ejection Mass Spectrometry (AEMS) and combines the speed of acoustic sampling with the selectivity of mass spectrometry. This platform has been designed for high throughput analysis of small and large molecules. The technology combines Acoustic Droplet Ejection (ADE), an Open Port Interface (OPI) and could be coupled with the SCIEX Triple Quad 6500+ system or the ZenoTOF 7600 system.
The Echo® MS+ system comprises of an open-port interface (OPI) and acoustic droplet ejection (ADE) module which could be coupled with a mass spectrometer. The mass spectrometer could either be a SCIEX Triple Quad 6500+ system or the ZenoTOF 7600 system. This non-liquid chromatography based; high-throughput screening platform enables rapid analysis of compounds at speeds of up to 1 sample/second.
The ability to consistently achieve reproducible results on many complex samples across multiple days is critical to a routine clinical laboratory. Laboratories relying on analytical instrumentation require stability and robustness to perform a variety of screening and confirmatory assays with confidence. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become the preferred analytical method in the clinical laboratory to reliably perform clinical testing as it provides best-in-class performance and reliability for the most challenging assays. LC-MS/MS offers the required levels of sensitivity and specificity for the detection and quantitation of molecules from complex biological samples, helping laboratories deliver highly accurate data for a variety of clinically relevant analytes across a wide range of assays.
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