Turbo charging your LC-MS/MS analysis

Nov 7, 2019 | Blogs, Technology | 0 comments

Would you like to take your LC-MS/MS analysis from 0 to 3 mL/min without the complexity of changing probes or splitting the LC flow? Then take a look into the Turbo V™ ion source from SCIEX.

Introduced more than 15 years ago, the Turbo V ion source continues to be a gold standard for rugged and user-friendly source design, for both ESI and APCI ionization. This shrewd architecture provides outstanding robustness and sensitivity when analyzing complex biological matrices with minimal maintenance. By merging two orthogonal streams of hot gas in the ESI region, efficient desolvation and hydrodynamic focusing of ions towards the sampling orifice is achieved.

For higher flow rate applications, this can translate to boosts in sensitivity of several orders of magnitude over unheated ESI sources. Desolvation is greatly improved without disrupting the curtain gas flow and greatly boosts the robustness of the entire system. Figure 1 schematically represents the heated gas flows within the Turbo V source.

The success of the Turbo V ion source over the years served as the inspiration and foundation upon which the more recently introduced IonDrive™ Turbo V and OptiFlow® Turbo V ion sources are designed. Each of these sources benefits from the use of orthogonally placed jets of heated gas directed at the liquid sample spray to enable even more efficiency of desolvation at different liquid flow regimes. While robustness and ruggedness were built in from the start, plug-and-play simplicity and a “just put it on, and it works” engineering is typical of all present-day SCIEX sources based on the Turbo V. Figure 2 highlights the wider heaters involved with the IonDrive Turbo V design for increased desolvation and thus higher sensitivity. The red square indicates the ‘sweet spot’ of standard Turbo V and the blue square highlights the bigger ‘sweet spot’ of IonDrive.

Key Benefits of the IonDrive Turbo V Source

  • High sensitivity for any matrix with enhanced ionization efficiency
    • Sensitivity is gained from hydrodynamic focusing of ESI droplets with a wider heater sweet spot in the Turbo V IonDrive source
  • Extraordinary robustness in performance providing higher productivity
    • The simple source architecture and orthogonal spray design with no complex spray path provides uniform temperature distribution and optimized curtain gas flow to improve robustness and ruggedness
  • High throughput analysis over a wide range of flow rates
    • With functional flow rate ranges from 5µl/min to 3 mL/min, the IonDrive Turbo V source is the perfect match for both micro flow HPLC applications with new low dispersion electrodes and analytical flow UHPLC flow rates, delivering unmatched desolvation and stability for the most demanding applications
  • Wider compound class coverage
    • The ability to quickly interchange ionization modes between APCI and ESI enables wider compound class coverage and easy and faster method development
  • Simple plug and play ion source design
    • A tool-free source design enables plug and play capability with almost zero maintenance

The recent introduction of OptiFlow technology allows the use of the Turbo V design to be translated to lower flow rate chromatography, such as nano and micro-flow rates. The benefit of lower flows can help with gains in sensitivity especially when you are ‘sample limited’. Significant research was done to develop a low flow source that is sensitive while maintaining high robustness and ease of use of higher flow sources. The result is an ion source, based on the legendary Turbo V design, with the ability to switch easily between nano and micro flow regimes. No need to change source, break vacuum and the system can be back up and running in minutes. Simple, robust and reliable. Figure 3 shows a picture of the Optiflow ion source based on the Turbo V geometry.Learn More about the Legendary Turbo V Ion Source >

To see more about the nearly 50 years of innovations of SCIEX mass spectrometry technology visit the Generation Quant Docu-Series.

Switching between different LCs that communicate through ethernet and setting up the correct module IP address

Different LC options are supported in SCIEX OS software and Analyst software. Those that communicate through ethernet differ in their default IP addresses. In this community post, we will detail the LAN setup, configuring the IP address and subnet mask for LC...

What are the differences between EPA methods 533 and 537.1?

With the risk of per- and polyfluoroalkyl substances (PFAS) contamination and accumulation in humans and wildlife on the rise, it is important to continuously improve and demonstrate capabilities for accurate and precise low-level quantification in research and...

Rescheduling a Schedule I substance, and the Delta-8 controversy

Did you know that in the US, drugs and other chemicals are classified into 5 distinct categories depending on the drug’s acceptable medical use and its potential for abuse or dependency?  Drugs federally classified as Schedule I substances by the US Drug Enforcement Administration (DEA) are considered to have the highest potential for abuse and for creating severe psychological and/or physical dependence. In addition to heroin, LSD and MDMA (ecstasy), cannabis is classified as a Schedule I substance in the Controlled Substance Act of 1970, which means it has no approved medical usage.

Posted by

Ashley Sage is the Senior Manager for Global Portfolio and Technology Strategic Marketing at SCIEX. He is responsible for looking after the strategic marketing campaigns for the product and technology portfolio. Most recently, Ashley was involved in the Generation Quant video creation and campaign. In his free time, Ashley enjoys golfing and spending time with his family.

0 Comments

Submit a Comment