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sMRM Pro Builder template tutorial

The sMRM Pro Builder template is an Excel-based tool that can help you implement large panels of analytes in your lab. The Excel sheet will take your preliminary experimental results and compute retention times, retention time window widths and dwell time weighting to optimize your targeted assay.

MRM method transfer from a SCIEX Triple Quad or QTRAP 6500+ system to the SCIEX 7500 system

MRM method transfer from a SCIEX Triple Quad or QTRAP 6500+ system to the SCIEX 7500 system

General recommendations when beginning method development Objective: The purpose of this document is to provide a quick reference for transferring MRM-based quantification methods from a SCIEX Triple Quad or QTRAP 6500+ system to a SCIEX 7500 system. While the best...

Identifying the unknown PFAS profile in firefighting foams/AFFF

Identifying the unknown PFAS profile in firefighting foams/AFFF

According to a recent study from Harvard University, the US EPA, and NIEHS, traditional targeted analysis techniques poorly characterize the PFAS composition of contemporary PFAS-based firefighting foams, know as aqueous film-forming foams (AFFF).  Using the EPA 533 PFAS drinking water method for the analyte list, the researchers found that targeted mass spectrometry methods accounted for <1% of organic fluorine content.  This is important because it demonstrates that targeted analysis methods miss nearly all the PFAS compounds in modern AFFF mixtures, thus underestimating the risk to human health and the environment.

How do I define the experimental design (the metadata) for my SWATH acquisition study within the OneOmics suite? What are the requirement for replicates?

How do I define the experimental design (the metadata) for my SWATH acquisition study within the OneOmics suite? What are the requirement for replicates?

In quantitative Omics research, the goal is to understand which analytes (protein or metabolite) are perturbed between experimental conditions; therefore we carefully design our studies to explore these questions. The algorithms used within the Assembler application...

Computing protein confidence with improved accuracy by reassessing peptide confidence during protein grouping

Computing protein confidence with improved accuracy by reassessing peptide confidence during protein grouping

ProteinPilot software 4.0 and higher releases include a new method for calculating protein confidences that improves reliability at the end of protein lists. Figure 1 shows a simulated example that demonstrates how more accurate protein confidence is computed. Figure...