The method of standard addition is a quantitative analysis approach used in situations where matrix effects from complex samples contributes to the analytical signal. This makes it impossible to compare the analytical signal between sample and standard using a traditional calibration curve approach. The standard is spiked directly into aliquots of the sample matrix. This technique is often used in cases where the matrix effects are very strong and no blank matrix is available for preparing a suitable matrix matched calibration. The sample with a background concentration of the target analyte is spiked with increasing amounts of known standard of that analyte and the subsequent standard addition curve and the response factor are used to extrapolate an unknown concentration in the original sample. MultiQuant™ Software is currently unable to perform this technique natively inside the software. It must be accomplished using a query architecture, which can be time consuming and prone to human error.
Standard addition processing simplified with SCIEX OS Software, SCIEX technical note RUO-MKT-07-7660-B.