Protocol for offline trap washing with NanoLC 425 system by Christie Hunter | Aug 7, 2021 | Data acquisition, LC, Life Science Research, Proteomics, SCIEX informationUsing the dual gradient system, the nanoLC 425 system can be configured such that the trap can be washed to waste rather than through the column and into the mass spectrometer. This workflow can be performed with either nanoflow or microflow chromatography. Figure 1....
Analysis of chiral amphetamines using differential mobility separation (DMS) by Pierre Negri | Aug 7, 2021 | Data acquisition, Differential Mobility Separation (DMS), SCIEX information, TechnologyMany compounds are challenging to analyze because they are isomers (have the same m/z) and also sometimes have the same HPLC retention time. And to make it even more complicated, sometimes there are two chiral forms that have different functions in biology, making it...
Separating cyclosporin isomers using differential mobility separation (DMS) by Pierre Negri | Aug 7, 2021 | Data acquisition, Differential Mobility Separation (DMS), SCIEX information, TechnologyCyclosporin A (CsA) has been a very successful immunosuppressant for preventing organ transplant rejection. It is a large cyclic peptide (11 residues, ~1200 Da) and there are numerous modifications of this drug, some of which are isomers. The challenge of analyzing...
Using Bio Tool Kit for interpreting peptide MS/MS data obtained using electron activated dissociation (EAD) by Christie Hunter | Aug 7, 2021 | Bio Tool Kit, Biopharma, Data processing, Data Visualization, Life Science Research, Proteomics, QTOF, SCIEX information, Technology, ZenoTOF 7600 systemPeptide MS/MS spectra obtained using either collision induced dissociation (CID) or electron activated dissociation (EAD) can be analyzed using the Peptide Fragments pane in Bio Tool Kit in SCIEX OS Software. First, open the EAD MS/MS spectrum that you want to...
Peptide fragmentation using electron activated dissociation (EAD) by Christie Hunter | Aug 7, 2021 | Bio Tool Kit, Biopharma, Data acquisition, Life Science Research, Proteomics, SCIEX information, Technology, ZenoTOF 7600 systemMost workflows in protein quantification and characterization by mass spectrometry have used collision-induced dissociation (CID). In the CID process, a peptide is selected in the first quadrupole of the instrument (Q1), then accelerated through a collision cell where...