Troubleshooting low flow LC – LC-MS peak area is lower than expected by Christie Hunter | Sep 20, 2021 | Data acquisition, LC, Life Science Research, Optimizing Performance, Proteomics, SCIEX informationSymptom: LC-MS peptide peak are is smaller than expected in PepCalMix Test Test: Use PepCalMix Infusion tests to determine if it is a problem with LC or MS Solution: First perform the SCIEX recommended performance tests using infusion on your MS system. If these...
Troubleshooting low flow LC – Spread of peptide elution is compressed or delayed by Christie Hunter | Sep 20, 2021 | Data acquisition, LC, Life Science Research, Optimizing Performance, Proteomics, SCIEX informationSymptom: The observed retention times of the peptides eluting are compressed, are not as spread out as expected, suggesting the elution gradient is compressed Test: Use PepCalMix LC-MS test to check the peptide elution time and spread Solution: Make sure you are...
Troubleshooting low flow LC – Loss of early eluting peptides during trap-elute workflow by Christie Hunter | Sep 20, 2021 | Data acquisition, LC, Life Science Research, Optimizing Performance, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemSymptom: Early eluting peptides are missing (based on monitoring PepCalMix LC-MS test or observing a change in the shape of the proteome TIC). Test: Use PepCalMix LC-MS test to monitor binding of early eluting peptides. There are a number of very hydrophilic peptides...
CloudTalk – Analytics for proteomics by Christie Hunter | Sep 20, 2021 | Data Visualization, How-to videos, Life Science Research, OneOmics, Proteomics, QTOF, ZenoTOF 7600 systemThis CloudTalk video (6:34min) describes the use of the Analytics app in OneOmics suite for evaluating the quality of your SWATH acquisition data. RUO-MKT-18-6582-A
CloudTalk – visualize your protein ID results within the ProteinPilot app in OneOmics suite by Alexandra Antonoplis | Sep 20, 2021 | Data Visualization, How-to videos, Life Science Research, OneOmics, Proteomics, QTOF, ZenoTOF 7600 systemThis CloudTalk video describes how to view ProteinPilot app search results using the Analytics and Browser applications within the ProteinPilot app. To perform a database search on your data using the ProteinPilot app, please watch CloudTalk – data processing...