GEN-MKT-18-7897-A
Jan 29, 2023 | Biopharma, Blogs, Echo® MS+ system, Pharma | 1 comment
You asked, we answered! With analysis speeds of at least 1 sample per second, the Echo® MS system has created a buzz in the industry. This is up to 50x faster than conventional LC-MS/MS. This revolutionary tool for drug discovery and development has led to many questions from scientists and researchers around the world. We answer the top 7 Echo® MS system questions here.
The sample ejection volume for the Echo® MS system is about 0.1% of what you are injecting into an LC-MS system. Therefore, you are putting many fewer contaminants into the system over the same period of time.
The SCIEX OS software controls the system. This allows us to automatically process the data and export the results to any visualization software or LIMS. The SCIEX OS API (Application Programming Interface) allows integration with automation software from robotics vendors.
What types of liquid handling systems can be interfaced upstream of the Echo MS system? And what plate/sample throughput rates are possible?
The system is compatible with any vendor’s robotics system that can manage 384- and 1536-well plates. The cycle time for 384-well plates is less than 10 minutes, and for 1536-well plates it is less than 30 minutes.
The total size of the system is approximately 1.3 meters x 1.4 meters. This does not include the acquisition computer (which can be located up to 2 meters from the system) or the 1 meter of service access required around the entire system.
For a 384-well plate, the minimum volume required in the well is 20 µL and for 1536-well plates the minimum volume required is 3 µL. This is to ensure efficient ejection of the sample droplet.
Do you have a question? Please submit your question today or add it below in the comments.
Learn more about what the Echo® MS system can do for your lab at sciex.com/echoms. You will be able to see inside the system, download the brochure, gain access to technical notes, watch the video and request a quote.
Finding the right information shouldn’t slow you down. Whether you’re troubleshooting your mass spec, learning something new, or optimizing performance, access to the right resources at the right moment makes all the difference.
As an analytical strategy, middle-down mass spectrometry (MS) workflows characterize biotherapeutic proteins by analyzing large, digested protein fragments or defined subunits, rather than fully intact proteins (top-down) or digested peptides (bottom-up). A middle-down strategy combines the strengths of top-down and bottom-up approaches by delivering high sequence coverage and structural specificity while maintaining relatively simple sample preparation. In practice, middle-down analysis enables accurate mass measurement, rapid sequence confirmation, and localization of key post-translational modifications (PTMs) on protein subunits that are directly relevant to product quality.
In biopharmaceutical development, sequence variants (SV) are considered an inherent risk of producing complex proteins in living systems. Sequence variants are unintended changes to the amino acid sequence of a biotherapeutic and can be caused by errors in transcription or translation in the host cell, or cell culture and process conditions. Detailed analysis of SVs is important in process and product development to ensure the drug’s safety and efficacy. Even low‑level sequence variants can have significant implications for product quality, safety, and efficacy, making their accurate detection and characterization a critical requirement across development, process optimization, and regulatory submission.
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