GEN-MKT-18-7897-A
Jan 1, 2016 | Blogs, Environmental / Industrial, Food / Beverage | 0 comments
When carrying out routine pesticide identification tests in your lab how simple is the process of identification, quantitation, and final confirmation from sample to sample? A reliable method designed to generate multiple data sets and confirm sample data in parallel with your test can save an awful lot of time and effort which is especially helpful as the demand for routine testing increases. In this poster talk, Detlev Schleuder, Support Manager for Food & Environmental Markets, explains how the new QTRAP® 6500+ system can optimize your laboratory’s output with this simple method.
A reliable method designed to generate multiple data sets and confirm sample data in parallel with your test can save an awful lot of time and effort which is especially helpful as the demand for routine testing increases. In this poster talk, Detlev Schleuder, Support Manager for Food & Environmental Markets, explains how the new 6500+ QTRAP® system can optimize your laboratory’s output with this simple method.Download the full poster >
Video Transcription (view video below)Welcome to RAFA 2015 in Prague again!
As you can imagine around 1,400 different pesticides are used worldwide, and demand is very high to measure them in a proper way – not forgetting the metabolites of the pesticides as well so there could even be thousands of different pesticides to test for.
Using the 6500+ either in Triple Quad or QTRAP mode, we have the chance of running two different approaches. By running it in Multiple Reaction Monitoring (MRM) mode to quantify, but also to confirm the data later on with the QTRAP technology in MS/MS mode.
QuEChERS extraction methodologySo what have we done here? We have performed a quick, easy, cheap, effective, rugged, safe (QuEChERS) extraction of fruits and vegetables and investigated more than 600 transitions by means of 300 different pesticides in positive and also negative ion mode, with very, very fast polarity switching of 5 milliseconds.
Over here you can see the results, and the results are brilliant with the 6500+ system, but also with the short transition or polarity switching time we have the chance to generate multiple data points over the different peaks
.
Information Dependent Acquisition (IDA)Nevertheless, sometimes the MRM ratios between the first and second MRM are not as good as wished for compared to normal ratios, but what can you do then?
Of course, then you need to do a confirmation of the compounds that you would like to find that are suspected of being false positive or false negative.
With this approach, on QTRAP mode you can perform parallel IDA data research, looking for specific MS/MS data and to confirm the data that you have acquired before and have suspected to be incorrect or correct – you will have a library search of the MS/MS data and have a proper confirmation of the data that you have acquired before. This is a (recommended) parallel approach that you can apply with your QTRAP 6500+.
To learn more simply click and download the complete pesticide resource kit >
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As an analytical strategy, middle-down mass spectrometry (MS) workflows characterize biotherapeutic proteins by analyzing large, digested protein fragments or defined subunits, rather than fully intact proteins (top-down) or digested peptides (bottom-up). A middle-down strategy combines the strengths of top-down and bottom-up approaches by delivering high sequence coverage and structural specificity while maintaining relatively simple sample preparation. In practice, middle-down analysis enables accurate mass measurement, rapid sequence confirmation, and localization of key post-translational modifications (PTMs) on protein subunits that are directly relevant to product quality.
In biopharmaceutical development, sequence variants (SV) are considered an inherent risk of producing complex proteins in living systems. Sequence variants are unintended changes to the amino acid sequence of a biotherapeutic and can be caused by errors in transcription or translation in the host cell, or cell culture and process conditions. Detailed analysis of SVs is important in process and product development to ensure the drug’s safety and efficacy. Even low‑level sequence variants can have significant implications for product quality, safety, and efficacy, making their accurate detection and characterization a critical requirement across development, process optimization, and regulatory submission.
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