GEN-MKT-18-7897-A
Jul 21, 2016 | Blogs, Food / Beverage | 0 comments
Food allergy is an immune-mediated, adverse reaction to an antigenic protein. Even limited exposure to an antigen can provoke a significant reaction in sensitive individuals, causing rashes, itching and swelling in the mouth, nausea, vomiting, and asthma. Additionally, food allergies are the leading cause of anaphylaxis, an acute, potentially deadly allergic reaction. The prevalence and severity of food allergies are rising, with approximately 150 million people suffering from food allergies worldwide.1, 2 Presently, there is no cure for food allergies, and sufferers must rely on the correct labeling of foods to avoid consuming allergens. Hence, the development of sensitive and accurate analytical methods to screen for the presence of allergens in food products is necessary for the prevention of potentially life-threatening health problems for allergy sufferers.
Enzyme-linked immunosorbent assays (ELISA) are the most commonly used tests for screening allergens. Although relatively quick and simple to perform, ELISA tests are limited in selectivity and susceptible to cross-reactivity, which can lead to false positive or false negative results. Additionally, most ELISA tests are capable of detecting only one allergen at a time, requiring multiple tests to screen for more than one allergen in a food sample. Therefore, a method that can unambiguously confirm and identify multiple allergens would be invaluable for food screening.
In our exciting solution to routine food allergen screening we have developed an LC-MS/MS method using the QTRAP® 4500 LC-MS/MS system that detects and screens 12 separate allergenic proteins simultaneously in a single injection.
The key benefits of why LC-MS/MS is the instrument of choice for food manufactures and testing facilities are:
Discover more about switching your allergen screening to LC-MS/MS >
In this toolkit you will access an overview brochure and a comprehensive technical note that details the method development steps and method performance in a variety of challenging natural and processed matrices.
References
For research use only. Not for use in diagnostic procedures
Trifluoroacetic acid (TFA) is emerging as one of the most concerning ultrashort-chain PFAS in Europe’s food supply – particularly in cereals, a staple consumed daily by millions. A report from PAN Europe reveals a widespread and largely unmonitored contamination trend that raises serious questions about food safety, regulatory blind spots, and future monitoring strategies.
PFAS analysis is complex, but expert guidance doesn’t have to be. In this episode of our ‘Ask the PFAS expert series’, we’re joined by Michael Scherer, Application Lead for Food and Environmental, to answer the most pressing questions in PFAS analysis. From why LC-MS/MS systems are the gold standard for analyzing diverse PFAS compounds, to which EU methods deliver reliable results for drinking water, and to practical steps to prevent contamination, Michael shares actionable insights to help laboratories achieve accuracy, consistency, and confidence in their workflows.
During an LC-MS/MS experiment, traditional fragmentation techniques like collision-induced dissociation (CID) have long been the gold standard. Electron-activated dissociation (EAD) is emerging as a transformative tool that enhances structural elucidation, particularly for complex or labile metabolites.
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